Wischnitzer Saul
Electron Microscope Laboratory, Department of Biology, Yeshiva University, New York City.
Wilhelm Roux Arch Entwickl Mech Org. 1970 Jun;166(2):150-172. doi: 10.1007/BF00576991.
The development of the mouse oocyte during the primordial, primary and secondary follicular growth stages was studied by means of the electron microscope.During the early stages of oocyte maturation, mitochondrial multiplication takes place along with an apparent temporary transition from round to oval shape. The internal structure of many of the mitochondria is altered by separation of membranes of a crista to form a vacuole. This enlarges to pear-shaped configurations and gradually it forms so large a structure as to result in compression of adjacent cristae, thereby altering the entire appearance of the organelle.Dense round bodies encapsulated by a single membrane are found in the cytoplasm of oocytes of primary follicles near the periphery. The Golgi complex appears in primary follicle oocytes as an aggregation of vesicles. Gradually the number of lamellae in the complexes increase and these organelles become more peripherally located. The "Balbiani yolk nuclei" apparently is represented by a conglomeration of Golgi complexes and are present only in primordial and young primary follicle oocytes.The endoplasmic reticulum appears in the early stages only as rough-surfaced vesicles. At later stages individual cisternae become prominent. Apparently, a modified form of E. R. appears during maturation of the secondary follicle oocyte.Multivesicular complexes, each consisting of two components, small vesicles and larger vesicles enclosing microvesicles (multivesicular bodies), were commonly found during all stages of oocyte growth. The secondary follicle oocytes frequently contain multilamellar bodies. These are commonly found in juxtaposition to the multivesicular complexes and also near the egg periphery and occasionally near the nuclear envelope.
通过电子显微镜研究了小鼠卵母细胞在原始卵泡、初级卵泡和次级卵泡生长阶段的发育情况。在卵母细胞成熟的早期阶段，线粒体进行增殖，同时明显地从圆形暂时转变为椭圆形。许多线粒体的内部结构因嵴膜分离形成液泡而发生改变。这个液泡扩大成梨形结构，并逐渐形成如此大的结构，以至于压迫相邻的嵴，从而改变了整个细胞器的外观。在初级卵泡卵母细胞外周的细胞质中发现有被单层膜包裹的致密圆形小体。高尔基体在初级卵泡卵母细胞中表现为小泡的聚集。复合物中的片层数量逐渐增加，这些细胞器变得更靠近外周。“巴尔比亚尼卵黄核”显然由高尔基体复合物聚集而成，仅存在于原始卵泡和年轻初级卵泡的卵母细胞中。内质网在早期仅表现为糙面小泡。在后期，单个的扁平囊变得明显。显然，在次级卵泡卵母细胞成熟过程中出现了一种内质网的变体形式。多囊复合体，每个由两个成分组成，即小泡和包裹微泡的较大泡（多囊体），在卵母细胞生长的所有阶段都普遍存在。次级卵泡卵母细胞经常含有多层小体。这些小体通常与多囊复合体并列存在，也靠近卵的外周，偶尔靠近核膜。
