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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Hadorn Ernst, Wetter-Schatzmann Dorothea

Zoologisch-vergleichend anatomisches Institut der Universität Zürich, Schweiz.

Wilhelm Roux Arch Entwickl Mech Org. 1974 Dec;174(4):337-340. doi: 10.1007/BF00579121.

Anterior halves of 6-hour-old embryos, in which the primordia of the salivary glands are not yet visibly segregated, have been transplanted into the abdomen of adult females. 2. Normal larval salivary glands differentiate in the adult hemolymph and attain the maximal degree of nuclear size due to polytenisation. 3. In the adult hemolymph, a culture period of 14 days is needed for full development whereas the same end-size is reachedin situ after 4 days. 4. The retardation in growth rate is accompanied by a reduction in cell numbers. 5. It is concluded that the adult hemolymph can provide all factors needed for the entire development of larval salivary glands. 6. The difference between the adult medium and the normal larval milieu which promotes a more rapid development might be only of a quantitative nature. Tentatively, we consider differences in hormonal concentrations.

将6小时大的胚胎前半部分（此时唾液腺原基尚未明显分离）移植到成年雌性动物的腹部。2. 正常幼虫唾液腺在成年动物的血淋巴中分化，并由于多线化而达到最大核尺寸。3. 在成年动物的血淋巴中，完全发育需要14天的培养期，而在原位4天后就能达到相同的最终大小。4. 生长速率的延迟伴随着细胞数量的减少。5. 得出的结论是，成年动物的血淋巴可以提供幼虫唾液腺整个发育所需的所有因素。6. 促进更快发育的成年培养基与正常幼虫环境之间的差异可能只是数量上的。我们初步认为是激素浓度的差异。

Hadorn Ernst, Wetter-Schatzmann Dorothea

Zoologisch-vergleichend anatomisches Institut der Universität Zürich, Schweiz.

Wilhelm Roux Arch Entwickl Mech Org. 1974 Dec;174(4):337-340. doi: 10.1007/BF00579121.

Anterior halves of 6-hour-old embryos, in which the primordia of the salivary glands are not yet visibly segregated, have been transplanted into the abdomen of adult females. 2. Normal larval salivary glands differentiate in the adult hemolymph and attain the maximal degree of nuclear size due to polytenisation. 3. In the adult hemolymph, a culture period of 14 days is needed for full development whereas the same end-size is reachedin situ after 4 days. 4. The retardation in growth rate is accompanied by a reduction in cell numbers. 5. It is concluded that the adult hemolymph can provide all factors needed for the entire development of larval salivary glands. 6. The difference between the adult medium and the normal larval milieu which promotes a more rapid development might be only of a quantitative nature. Tentatively, we consider differences in hormonal concentrations.

将6小时大的胚胎前半部分（此时唾液腺原基尚未明显分离）移植到成年雌性动物的腹部。2. 正常幼虫唾液腺在成年动物的血淋巴中分化，并由于多线化而达到最大核尺寸。3. 在成年动物的血淋巴中，完全发育需要14天的培养期，而在原位4天后就能达到相同的最终大小。4. 生长速率的延迟伴随着细胞数量的减少。5. 得出的结论是，成年动物的血淋巴可以提供幼虫唾液腺整个发育所需的所有因素。6. 促进更快发育的成年培养基与正常幼虫环境之间的差异可能只是数量上的。我们初步认为是激素浓度的差异。