Zamenhof Stephen
Departments of Microbiology and Immunology, and Biological Chemistry, Mental Retardation Research Center, and Brain Research Institute, U.C.L.A. School of Medicine, 90024, Los Angeles, Ca., USA.
Wilehm Roux Arch Dev Biol. 1976 Mar;180(1):1-8. doi: 10.1007/BF00848881.
Experimental chick embryos were incubated at 37.5°C till day 7 and after day 10, and at 40.5°C on days 7-10; their optic lobes and cerebral hemispheres at day 10 and at hatching were compared with controls incubated at 37.5°C only. Cell numbers at day 10 were directly counted by a new method involving formalin fixation and cell disaggregation by gentle sonication. At hatching, body weights, organ weights and organ DNA (cell numbers) were the same in experimentals and in controls, for both optic lobes and cerebral hemispheres, though the protein contents were significantly higher in experimentals. However, at 10 days (end of neuron proliferation) the weights and the cell numbers in experimentals were significantly higher. Two possible explanations have been offered: 1. Elevated neuron population in experimental animals at day 10 is followed by their elevated death rate, or 2. The increment in neuron number is permanent but at hatching it is overshadowed by the population of other cells.
将实验用鸡胚在37.5°C下孵育至第7天，之后在第10天后在40.5°C下孵育，在第7至10天在40.5°C下孵育；将它们在第10天和孵化时的视叶和大脑半球与仅在37.5°C下孵育的对照进行比较。通过一种新方法直接计数第10天的细胞数量，该方法包括福尔马林固定和通过温和超声处理使细胞解离。在孵化时，实验组和对照组的体重、器官重量和器官DNA（细胞数量）在视叶和大脑半球方面均相同，尽管实验组的蛋白质含量显著更高。然而，在第10天（神经元增殖结束时），实验组的重量和细胞数量显著更高。提出了两种可能的解释：1. 实验动物在第10天神经元数量增加后，其死亡率升高；或者2. 神经元数量的增加是永久性的，但在孵化时被其他细胞群体掩盖。
