Kieny Madeleine, Pautou Marie-Paule
Laboratoire de Zoologie et Biologie animale, Equipe de Recherche associée au CNRS no 621, Université scientifique et médicale de Grenoble, Boîte postale no 53-Centre de Tri, F-38041, Grenoble-Cedex, France.
Wilehm Roux Arch Dev Biol. 1977 Sep;183(3):177-191. doi: 10.1007/BF00867319.
Deficient limb buds composed of prospective stylopod and autopod are able to regulate the missing intercalary zeugopod, the origin of which was investigated by heterospecific quail/chick recombinants. The associations of quail prospective autopod and chick prospective stylopod failed to regulate. The reverse combination of chick prospective autopod grafted onto a quail prospective stylopod gave rise to a three-segmented limb. In 13 out of 16 cases the regulated zeugopod was made up of both chick and quail cells. Chick cells were located predominantly along the postaxial half of the zeugopod, while the quail cells made up most of its preaxial half. In two cases, the intercalary zeugopod consisted exclusively of chick cells originating from the tip and in one case of quail cells originating from the base.These results demonstrate that during the regulative processes, the prospective values of some of the original stylopodial and autopodial cells have been shifted along the proximo-distal axis, towards the expression of more distal as well as of more proximal structures.Heteropolar stylo-autopodial or zeugo-autopodial recombinants, in which the proximo-distal axis of the base was reversed with respect to that of the tip, were unable to regulate the pattern defects and thus revealed the importance of concordant p-d polarity for regulative processes to take place between abutted tissues.
由预期的上肢带骨和手足组成的肢体芽缺失时，能够调节缺失的中间段zeugopod，通过异种鹌鹑/鸡重组体研究了其起源。鹌鹑预期的手足与鸡预期的上肢带骨的组合未能实现调节。将鸡预期的手足移植到鹌鹑预期的上肢带骨上的反向组合产生了一个三段式肢体。在16例中的13例中，调节后的zeugopod由鸡和鹌鹑细胞组成。鸡细胞主要位于zeugopod的轴后半部，而鹌鹑细胞构成了其轴前半部的大部分。在两例中，中间段zeugopod仅由源自尖端的鸡细胞组成，在一例中由源自基部的鹌鹑细胞组成。这些结果表明，在调节过程中，一些原始上肢带骨和手足细胞的预期值已沿近远轴发生转移，朝着更多远端以及更多近端结构的表达方向发展。异极上肢带骨-手足或zeugopod-手足重组体，其中基部的近远轴相对于尖端的近远轴是反向的，无法调节模式缺陷，从而揭示了一致的近远极性对于相邻组织之间发生调节过程的重要性。
