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红头丽蝇(双翅目,丽蝇科)卵巢蛋白质合成的差异表明其性别是预先确定的。

Difference in protein synthesis of ovaries indicates predetermined sex inChrysomya rufifacies (Diptera, Calliphoridae).

作者信息

Kirchhoff Christiane

机构信息

Biologiezentrum, Zoologisches Institut der Universität, Olshausenstraße 40-60, D-2300, Kiel, Federal Republic of Germany.

出版信息

Rouxs Arch Dev Biol. 1986 Apr;195(3):182-185. doi: 10.1007/BF02439436.

DOI:10.1007/BF02439436
PMID:28305253
Abstract

Sex in the monogenic blowflyChrysomya rufifacies is under the control of a germ-line autonomous maternal effect sex realizer. In order to identify the ovarian poly(A)RNA that might be related to sex predetermination, we analysed the patterns of cell-free translation products of total poly(A)RNA from female and male predetermined ovaries and oocytes. During vitellogenesis we observed one transient sex-linked difference in a stable pattern composed of more than 800 in vitro translated proteins. This difference, however, was no longer detectable in mature oocytes.

摘要

单基因丽蝇红头丽蝇的性别受生殖系自主母体效应性别决定因子的控制。为了鉴定可能与性别预决定相关的卵巢多聚腺苷酸RNA,我们分析了来自雌性和雄性预定卵巢及卵母细胞的总多聚腺苷酸RNA的无细胞翻译产物模式。在卵黄发生过程中,我们在由800多种体外翻译蛋白质组成的稳定模式中观察到一种短暂的性别连锁差异。然而,这种差异在成熟卵母细胞中不再可检测到。

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2
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引用本文的文献

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本文引用的文献

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Electrophoretic analysis of newly synthesized and stored maternal RNA during oogenesis ofCalliphora erythrocephala (Dipt.).红头丽蝇(双翅目)卵子发生过程中新合成和储存的母体RNA的电泳分析。
Wilehm Roux Arch Dev Biol. 1981 Nov;190(6):331-338. doi: 10.1007/BF00863270.
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Low variability of the protein species synthesized byDrosophila melanogaster embryos.黑腹果蝇胚胎合成的蛋白质种类变异性低。
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Proteins of the Drosophila melanogaster male reproductive system: two-dimensional gel patterns of proteins synthesized in the XO, XY, and XYY testis and paragonial gland and evidence that the Y chromosome does not code for structural sperm proteins.黑腹果蝇雄性生殖系统的蛋白质:XO、XY和XYY睾丸及旁腺中合成蛋白质的二维凝胶图谱以及Y染色体不编码精子结构蛋白的证据
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A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.一种用于检测聚丙烯酰胺凝胶中氚标记蛋白质和核酸的胶片检测方法。
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