Blüthmann Horst, Illmensee Karl
Department of Animal Biology, University of Geneva, CH-1224, Geneva, Switzerland.
Wilehm Roux Arch Dev Biol. 1981 Nov;190(6):374-378. doi: 10.1007/BF00863277.
The nonhistone protein pattern of four murine teratocarcinomas with different capacities for differentiation were compared: a multidifferentiated teratocarcinoma OTT2289, a nondifferentiated teratocarcinoma OTT2158, a teratocarcinoma-derived rhabdomyosarcoma TDR114, and a teratocarcinoma-derived neuroblastoma TDN2151. Their nonhistone proteins (NHP) were separated by differential salt extraction and hydroxyapatite chromatography into three fractions, NHP-I, NHP-II and NHP-III. Comparison of the NHP fractions by twodimensional gel electrophoresis in combination with a sensitive silver staining method reveals that there are several tumour line specific proteins in each NHP fraction. We suggest that specific NHP, which can be used as biochemical markers for each of the four investigated tumour lines, may be involved in cell lineage specific control of gene expression.
一种多分化畸胎瘤OTT2289、一种未分化畸胎瘤OTT2158、一种源自畸胎瘤的横纹肌肉瘤TDR114和一种源自畸胎瘤的神经母细胞瘤TDN2151。通过差异盐提取和羟基磷灰石色谱法将它们的非组蛋白（NHP）分离为三个部分，即NHP-I、NHP-II和NHP-III。结合灵敏的银染法，通过二维凝胶电泳对NHP部分进行比较，结果显示每个NHP部分都有几种肿瘤细胞系特异性蛋白。我们认为，特定的NHP可能参与基因表达的细胞系特异性调控，可作为所研究的四种肿瘤细胞系各自的生化标志物。
