Blüthmann H
Eur J Cell Biol. 1985 Sep;38(2):254-62.
The transition in mouse teratocarcinomas of pluripotential stem cells to histogenetically determined ones of the neurogenic cell lineage was analysed at the total cellular protein level by two-dimensional gel electrophoresis. The change in morphology and function was found to be reflected by extensive shifts in the protein synthesis patterns. From a total of about 1000 resolved polypeptides that are synthesized in embryoid bodies of a multidifferentiating teratocarcinoma, about 12% (117 proteins) are not found or only present at a very much reduced level in two teratocarcinoma-derived neuroblastomas. On the other hand, the change in phenotype is accompanied by the de novo or greatly enhanced synthesis of another 69 proteins (about 7%) in the neurogenic tumors. In a screening for differentiation-specific proteins this set was compared with the protein synthesized in neural tissue of maturing brain and muscle tissue of developing limbs at three different postimplantation stages covering the onset of organogenesis. This comparison disclosed that a great portion of the differentiation-related proteins (26 proteins) are synthesized in both brain and muscle and are probably required by a differentiated cell in general like cytostructural proteins. Seventeen polypeptides, however, are synthesized in a cell type specific manner. In particular, 4 proteins that are synthesized in both neurogenic tumors and in brain but not in muscle tissue were tentatively called nerve-specific proteins (NSP) and are the most promising in further analyses of differentiation-specific proteins.
通过二维凝胶电泳在总细胞蛋白水平上分析了小鼠畸胎癌中多能干细胞向神经源性细胞谱系的组织发生学确定细胞的转变。发现形态和功能的变化反映在蛋白质合成模式的广泛改变上。在一个多分化畸胎癌的胚状体中总共约1000种可分辨的多肽中，约12%（117种蛋白质）在两种畸胎癌衍生的神经母细胞瘤中未被发现或仅以非常低的水平存在。另一方面，表型的变化伴随着神经源性肿瘤中另外69种蛋白质（约7%）的从头合成或大量增加的合成。在对分化特异性蛋白质的筛选中，将这一组蛋白质与在器官发生开始的三个不同植入后阶段成熟脑的神经组织和发育肢体的肌肉组织中合成的蛋白质进行了比较。这种比较表明，很大一部分与分化相关的蛋白质（26种蛋白质）在脑和肌肉中都有合成，并且可能像细胞结构蛋白一样是分化细胞普遍需要的。然而，有17种多肽是以细胞类型特异性方式合成的。特别是，在神经源性肿瘤和脑中都有合成但在肌肉组织中没有合成的4种蛋白质被暂时称为神经特异性蛋白质（NSP），它们在进一步分析分化特异性蛋白质方面最具前景。
