Flavin M, Ton That H, Deparis P, Duprat A M
Laboratoire de Biologie générale, Université Paul Sabatier, 118 route de Narbonne, F-31062, Toulouse Cedex, France.
Unité de Recherches sur les Anémies, Inserum U 91, Hopital H. Mondor, F-94010, Creteil, France.
Wilehm Roux Arch Dev Biol. 1982 May;191(3):185-190. doi: 10.1007/BF00848334.
In order to study the cellular distribution of larval and adult hemoglobins during larval development ofPleurodeles waltlii a double specific immunofluorescent labelling technique was developed.Rabbit antibodies specific for larval and adult hemoglobin components were prepared and conjugated with tetramethyl-rhodamine isothiocyanate for the anti-larval antibodies and fluorescein isothiocyanate for the anti-adult hemoglobin antibodies.Both simultaneous and sequential staining with the two types of fluorescent antibodies indicated that larval and adult hemoglobins were never observed within the same erythrocyte during development. The results provide evidence that two distinct cell populations exist, one synthesizing exclusively larval hemoglobins which is progressively replaced by the other one synthesizing exclusively adult hemoglobins. It remains to be determined if these two populations arise from two distinct types of stem cells (adult and larval) or from the same stem cell type.
为了研究滑蹠螈幼体发育过程中幼体血红蛋白和成体血红蛋白的细胞分布，开发了一种双特异性免疫荧光标记技术。制备了针对幼体血红蛋白成分和成体血红蛋白成分的兔抗体，抗幼体血红蛋白抗体与异硫氰酸四甲基罗丹明偶联，抗成体血红蛋白抗体与异硫氰酸荧光素偶联。用这两种荧光抗体同时染色和顺序染色均表明，在发育过程中，从未在同一红细胞内观察到幼体血红蛋白和成体血红蛋白。结果证明存在两个不同的细胞群体，一个群体专门合成幼体血红蛋白，逐渐被另一个专门合成成体血红蛋白的群体所取代。这两个群体是来自两种不同类型的干细胞（成体和幼体）还是来自同一干细胞类型，仍有待确定。
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