Alter B P, Goff S C, Efremov G D, Gravely M E, Huisman T H
Br J Haematol. 1980 Apr;44(4):527-34. doi: 10.1111/j.1365-2141.1980.tb08706.x.
Separation of globin chains by electrophoresis provides a simple and rapid method for the determination of the G gamma/A gamma ratio in human fetal haemoglobin, and of biosynthetic rates of the globin chains. Whole haemolysates were analysed by electrophoresis on polyacrylamide gels in urea, acetic acid and Triton X-100. Electrophoresis of haemolysates from newborn infants led to four bands: A gamma, G gamma, beta and alpha. The identity of these bands was indicated by examination of haemoglobins of known globin chain composition. In 15 samples, the % G gamma was similar by Triton gels and by amino acid analysis of the gamma CB-3 peptide. Some mutant globin chains were also separable with the electrophoretic technique. Triton gel electrophoresis provides rapid analysis of very small amounts of haemoglobin, and permits examination of globin chain composition as well as globin synthetic ratios.
通过电泳分离珠蛋白链为测定人胎儿血红蛋白中的Gγ/Aγ比率以及珠蛋白链的生物合成速率提供了一种简单快速的方法。通过在含有尿素、乙酸和 Triton X - 100的聚丙烯酰胺凝胶上进行电泳来分析全溶血产物。对新生儿溶血产物进行电泳产生了四条带：Aγ、Gγ、β和α。通过检查已知珠蛋白链组成的血红蛋白来确定这些条带的身份。在15个样本中，通过Triton凝胶和γCB - 3肽的氨基酸分析得到的Gγ百分比相似。一些突变珠蛋白链也可用电泳技术分离。Triton凝胶电泳可对极少量的血红蛋白进行快速分析，并可检查珠蛋白链组成以及珠蛋白合成比率。
