Transgenic markers for mammalian chimeras.
作者信息
Thomson James Alexander, Solter Davor
机构信息
The Wistar Institute, 36th at Spruce Street, 19104, Philadelphia, PA, USA.
出版信息
Rouxs Arch Dev Biol. 1988 Mar;197(2):63-65. doi: 10.1007/BF00375928.
PMID:28305597
Abstract
We describe the construction of aggregation chimeras between normal and transgenic embryos containing multiple copies of mouse β-globin genes. The transgenic component of the chimeras is then detected in tissue sections by a DNA-DNA in situ hybridization technique, using a biotinylated DNA β-globin probe and an avidin-linked alkaline phosphatase detection system. The general advantages of transgenic markers for chimeras are discussed.
摘要
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