Soussi T, Caron de Fromentel C, Méchali M, May P, Kress M
Unité d'Oncologie Moléculaire, IRSC, Villejuif, France.
Oncogene. 1987 Mar;1(1):71-8.
A Xenopus laevis oocyte cDNA library was screened with a murine p53 cDNA probe for the presence of p53-related clones. Several such clones were isolated and analysed. The nucleotide sequence of the largest cDNA clone (2.2 kb) showed a high degree of homology with the human (68%) and murine (70%) p53 coding sequences. This clone contains a single large open-reading frame, coding for a protein of 363 amino acids, which is 51% homologous to human p53 and 57% homologous to murine p53. Furthermore, five highly conserved internal regions were found in all three proteins. The three proteins have a highly similar amino acid composition (including, notably, the presence of a high proportion of proline residues), and they display a comparable distribution of charged amino acids and hydropathic index profile. The in vitro transcription-translation products of the X. laevis clone were successfully immunoprecipitated by human anti-p53 sera, demonstrating that there is at least one epitope in common between the X. laevis protein and human, and possibly murine, p53.
用鼠p53 cDNA探针筛选非洲爪蟾卵母细胞cDNA文库，以寻找与p53相关的克隆。分离并分析了几个这样的克隆。最大的cDNA克隆（2.2 kb）的核苷酸序列与人（68%）和鼠（70%）的p53编码序列具有高度同源性。该克隆包含一个单一的大开放阅读框，编码一个由363个氨基酸组成的蛋白质，该蛋白质与人p53的同源性为51%，与鼠p53的同源性为57%。此外，在所有这三种蛋白质中都发现了五个高度保守的内部区域。这三种蛋白质具有高度相似的氨基酸组成（特别是含有高比例的脯氨酸残基），并且它们显示出相当的带电荷氨基酸分布和疏水指数图谱。非洲爪蟾克隆的体外转录-翻译产物被人抗p53血清成功免疫沉淀，表明非洲爪蟾蛋白质与人以及可能与鼠的p53之间至少有一个共同的表位。
