Fleig Richard
Institut für Biologic I (Zoologie), Albertstr. 21 a, D-79104, Freiburg, Germany.
Rouxs Arch Dev Biol. 1994 Jan;203(4):227-229. doi: 10.1007/BF00636338.
Segmentation in the head of the embryo of the Colorado beetleLeptinotarsa decemlineata is described on the basis of anti-engrailed (en) immunostaining of germ band stages. Six segmental units can be identified with this technique. Three segmentalen stripes can be distinguished in the gnathal region, a weak stripe interrupted medially shows the intercalary segment rudiment, a pair of oblique stripes indicate the antennal segment, and one pair of preantennalen spots are taken to indicate a sixth segment. In the broad head lobes of the beetle the spacing of the six segmental units as demarcated byen regions is similar to that in other parts of the germ band. The results are discussed with respect to old and new data concerning the number of head segments and origin of the compound eye in insects.
基于对胚带期的抗 engrailed（en）免疫染色，描述了科罗拉多马铃薯甲虫Leptinotarsa decemlineata胚胎头部的分节情况。用这种技术可以识别出六个节段单元。在颚区可以区分出三条节段性条纹，一条在中间中断的弱条纹显示了间节的雏形，一对斜条纹表示触角节，一对触角前斑点被认为表示第六节。在甲虫宽阔的头部叶中，由en区域划定的六个节段单元的间距与胚带其他部分的间距相似。结合关于昆虫头部节段数量和复眼起源的新旧数据对结果进行了讨论。
