Bidmon Hans-Jürgen, Granger Noelle Audrey, Stumpf Walter Erich
Department of Cell Biology and Anatomy, University of North Carolina at Chapel Hill, 522 Taylor Hall, 27599-7090, Chapel Hill, NC, USA.
Rouxs Arch Dev Biol. 1991 Aug;200(3):149-155. doi: 10.1007/BF00190234.
The presence of c-fos, a marker for cell activation, was investigated in cerebral neurons actively expressing ecdysteroid receptors during larval-pupal development in the tobacco hornworm, Manduca sexta. Colocalization was accomplished by ecdysteroid autoradiography using the tritiated high affinity 20-hydroxyecdysone agonist ponasterone A and immunocytochemistry with an antibody to a peptide sequence which is highly conserved in both human and murine c-fos. Immunoreactivity to a c-fos-like protein(s) was present in nuclei of many neurons of all the developmental stages examined. However, with the exception of the optic lobe, cells expressing nuclear ecdysteroid receptors were more immunoreactive than non-ecdysteroid-binding neurons. These data suggest that ecdysteroid-induced gene activation and translation may involve c-fos expression.
在烟草天蛾（Manduca sexta）幼虫-蛹发育过程中，对积极表达蜕皮甾体受体的脑神经元中细胞激活标记物c-fos的存在情况进行了研究。通过使用氚标记的高亲和力20-羟基蜕皮酮激动剂ponasterone A进行蜕皮甾体放射自显影以及使用针对在人和鼠c-fos中高度保守的肽序列的抗体进行免疫细胞化学来实现共定位。在所检查的所有发育阶段的许多神经元细胞核中都存在对c-fos样蛋白的免疫反应性。然而，除视叶外，表达核蜕皮甾体受体的细胞比不结合蜕皮甾体的神经元具有更高的免疫反应性。这些数据表明，蜕皮甾体诱导的基因激活和翻译可能涉及c-fos的表达。
