Boyan George S, Williams J Leslie D, Reichert Heinrich
Laboratory of Neurobiology, Institute of Zoology, University of Basel, Rheinsprung 9, CH-4051, Basel, Switzerland.
Rouxs Arch Dev Biol. 1995 Sep;205(1-2):45-53. doi: 10.1007/BF00188842.
We have studied a group of midline cells in the embryonic brain of the grasshopper by using immunocytochemical and intracellular dye injection techniques. This cluster of midline cells differentiates between the pars intercerebralis lobes of the protocerebrum during early embryogenesis, and is composed of putative midline progenitors as well as neuronal and glial cells. Annulin immunoreactive glial processes surround the borders of the midline cell cluster and also form a network of processes extending from there to the borders of proliferative clusters in the brain hemispheres. Among the cells that derive from the midline cluster are two bilaterally symmetrical pairs of identified primary commissure pioneer neurons. By navigating along the glial bound borders of the midline proliferative cluster, the axons of these pioneers establish an initial axonal bridge across the brain midline. This analysis identifies a glial-bound midline proliferative cluster in the brain and shows that neuronal and glial cells of this cluster are closely associated with neurons pioneering the primary brain commissure. Comparable features of midline cells in the ventral ganglia and similarities to other proliferative clusters in the brain hemispheres are discussed.
我们运用免疫细胞化学和细胞内染料注射技术，对蝗虫胚胎大脑中的一组中线细胞进行了研究。这一中线细胞簇在胚胎发育早期于前脑的脑间叶之间分化形成，由假定的中线祖细胞以及神经元和神经胶质细胞组成。环蛋白免疫反应性神经胶质突起环绕着中线细胞簇的边界，并且还形成了一个从那里延伸至脑半球增殖簇边界的突起网络。源自中线簇的细胞中有两对双侧对称的已鉴定的初级连合先驱神经元。通过沿着中线增殖簇的神经胶质边界导航，这些先驱神经元的轴突在脑中线建立了最初的轴突桥。该分析确定了大脑中一个由神经胶质界定的中线增殖簇，并表明该簇的神经元和神经胶质细胞与开拓初级脑连合的神经元密切相关。文中还讨论了腹神经节中线细胞的可比特征以及与脑半球其他增殖簇的相似性。
