在海鞘柄海鞘卵母细胞的生发泡内检测到一种83 kDa的胚胎型核抗原。

An 83-kDa embryonic-type nuclear antigen is detected within the germinal vesicles of oocytes of the ascidianHalocynthia roretzi.

作者信息

Fujiwara Shigeki, Satoh Noriyuki

机构信息

Department of Zoology, Faculty of Science, Kyoto University, Sakyo-ku, 606, Kyoto, Japan.

Asamushi Marine Biological Station, Tohoku University, 039-34, Aomori, Japan.

出版信息

Rouxs Arch Dev Biol. 1990 Apr;199(4):207-211. doi: 10.1007/BF01682079.

DOI:10.1007/BF01682079

PMID:28306105

Abstract

Monoclonal antibodies were raised against germinal vesicles which were isolated from fully grown oocytes of the ascidianHalocynthia roretzi. Immunoblot analyses revealed that one of the antibodies, designated Hgv-2, recognized a single band with a molecular weight of about 83 kDa. The antibody, visualized by indirect immunohistochemistry, reacted only with the germinal vesicles of oocytes and did not react with test cells, follicle cells, and other somatic cells of the gonad. During embryogenesis the antigenicity was found in interphase nuclei of all embryonic cells. The antibody did not react with chromosomes or the mitotic apparatus. The antigenicity was retained by interphase nuclei of larval cells, but it disappeared from nuclei of juveniles about 7 days after metamorphosis.

摘要

制备了针对从海鞘罗雷氏海鞘完全成熟的卵母细胞中分离出的生发泡的单克隆抗体。免疫印迹分析显示，其中一种名为Hgv-2的抗体识别出一条分子量约为83 kDa的单一带。通过间接免疫组织化学观察到，该抗体仅与卵母细胞的生发泡反应，而不与性腺的测试细胞、卵泡细胞和其他体细胞反应。在胚胎发育过程中，在所有胚胎细胞的间期核中发现了抗原性。该抗体不与染色体或有丝分裂装置反应。幼虫细胞的间期核保留了抗原性，但在变态后约7天，幼体的核中抗原性消失。