Dreyer Christine, Singer Helen, Hausen Peter, Müller Ursula, Siegel Elisabeth
Max-Planck-Institut für Virusforschung, Spemannstr. 35, D-7400, Tübingen, Germany.
Wilehm Roux Arch Dev Biol. 1981 Jul;190(4):197-207. doi: 10.1007/BF00848303.
A library of hybridoma cell lines has been established which produce monoclonal antibodies against antigens from the germinal vesicle ofXenopus laevis oocytes. Many of the antigens are also found in the nuclei ofXenopus embryonic cells in culture. The fate of two of these antigens during embryogenesis was traced by immunofluorescence on embryo and tadpole sections. Early in development these antigens appear to be evenly distributed in the nuclei of all cells. In later stages they gradually disappear from most embryonic structures but are strongly accumulated in the nuclei of some specific cell types and organs.
已经建立了一个杂交瘤细胞系文库，该文库产生针对非洲爪蟾卵母细胞生发泡抗原的单克隆抗体。在培养的非洲爪蟾胚胎细胞的细胞核中也发现了许多这些抗原。通过对胚胎和蝌蚪切片进行免疫荧光，追踪了其中两种抗原在胚胎发育过程中的命运。在发育早期，这些抗原似乎均匀分布在所有细胞的细胞核中。在后期阶段，它们逐渐从大多数胚胎结构中消失，但在某些特定细胞类型和器官的细胞核中强烈积累。
