Kozarac Z, Dhathathreyan A, Möbius D
Center for Marine Research, Rudjer Boskovic Institute, Zagreb, Croatia, Yugoslavia.
FEBS Lett. 1988 Mar 14;229(2):372-6. doi: 10.1016/0014-5793(88)81159-1.
The uptake of cytochrome c by charged and neutral lipid monolayers was studied by using reflection spectroscopy. The method was shown to be a very sensitive and useful technique in studies of lipid-protein interactions. It was found that cytochrome c is preferentially bound to monolayers of negatively charged monolayers in the solid phase. Polarized light under oblique incidence was used to determine the average orientation of chromophores in cytochrome c bound to lipid monolayer. The transition moments of chromophore are oriented parallel to the monolayer plane.
通过反射光谱法研究了带电和中性脂质单层对细胞色素c的摄取。该方法在脂质-蛋白质相互作用研究中被证明是一种非常灵敏且有用的技术。研究发现，细胞色素c优先结合于固相带负电荷的单层。使用斜入射的偏振光来确定结合在脂质单层上的细胞色素c中发色团的平均取向。发色团的跃迁矩平行于单层平面取向。
