Raevskaia G B, Varshaver N B, Shapiro N I
Genetika. 1987 Dec;23(12):2196-203.
Fifteen revertant clones exhibiting contact inhibition, one of the typical characteristics of normal cells, were studied after treatment of spontaneously transformed Chinese hamster fibroblasts with SV40. The clones proved to be partial revertants, as regards to other properties of the normal phenotype--loss of the ability to grow in a medium with a low serum content and anchorage-dependence. Viral DNA was detected in all revertant clones. The expression of T-antigen--the product of viral oncogene, was observed in 13 of 15 revertants analyzed. The study of SV40 "rescued" from several revertants in permissive monkey cells has shown that the virus is non-defective. In 7 clones, reversion was accompanied with polyploidization. In the cases, reversion could be due to changes in the balance between oncogenes and suppressor genes (anti-oncogenes). The possibility of induction by SV40 of mutations in anti-oncogenes suppressing the expression of both cellular and viral oncogenes is discussed. It is suggested that reversion to the normal phenotype in clones with a near-diploid karyotype could result from such virus-induced suppressor mutations.
在用SV40处理自发转化的中国仓鼠成纤维细胞后，对15个表现出接触抑制（正常细胞的典型特征之一）的回复克隆进行了研究。就正常表型的其他特性而言，这些克隆被证明是部分回复体——在低血清含量培养基中生长的能力丧失以及锚定依赖性丧失。在所有回复克隆中均检测到病毒DNA。在分析的15个回复体中的13个中观察到了病毒癌基因产物T抗原的表达。对从允许性猴细胞中的几个回复体中“拯救”出的SV40的研究表明，该病毒无缺陷。在7个克隆中，回复伴随着多倍体化。在这些情况下，回复可能是由于癌基因和抑癌基因（抗癌基因）之间平衡的变化。讨论了SV40诱导抑制细胞和病毒癌基因表达的抗癌基因突变的可能性。有人提出，具有近二倍体核型的克隆回复到正常表型可能是由这种病毒诱导的抑制突变引起的。
