Cardini J F, Santos R A, Martins A S, Machado R P, Alzamora F
Department of Physiology and Biophysics, Federal University of Minas Gerais, Belo Horizonte, Brazil.
Hypertension. 1988 Feb;11(2 Pt 2):I66-8. doi: 10.1161/01.hyp.11.2_pt_2.i66.
The stop-flow technique was used to determine the site of entry of kininase II into tubular fluid in dogs. Stop-flow patterns were constructed for kininase II, p-aminohippurate, sodium, and potassium. The proximal tubule was localized by the peak of p-aminohippurate concentration and the distal tubule by the minimum sodium concentration. In the stop-flow pattern for kininase II, three peaks (a, b, and c) were observed. A main peak (a), located 2.25 +/- 0.45 ml distal to the p-aminohippurate peak (p less than 0.01) and 3.75 +/- 0.31 ml proximal to the minimum sodium concentration (p less than 0.001), was observed in all experiments. Peak c, located 2.6 +/- 0.4 ml (p less than 0.01) proximal to the p-aminohippurate peak, was observed in five dogs. Peak b appeared in five dogs and was always located 2.0 ml distal to the minimum sodium concentration. This peak was coincident with the potassium peak. Only two of eight experiments showed all three peaks. These results showed that the major kininase II entry into the tubular fluid is near the p-aminohippurate peak and that distal entry occurred in 63% of the dogs.
采用停流技术来确定犬体内激肽酶II进入肾小管液的部位。构建了激肽酶II、对氨基马尿酸、钠和钾的停流模式图。近端小管通过对氨基马尿酸浓度峰值定位，远端小管通过最低钠浓度定位。在激肽酶II的停流模式图中，观察到三个峰值（a、b和c）。在所有实验中均观察到一个主峰（a），它位于对氨基马尿酸峰值远端2.25±0.45 ml处（p<0.01），且位于最低钠浓度近端3.75±0.31 ml处（p<0.001）。在五只犬中观察到峰值c，它位于对氨基马尿酸峰值近端2.6±0.4 ml处（p<0.01）。峰值b出现在五只犬中，且总是位于最低钠浓度远端2.0 ml处。该峰值与钾峰值重合。八次实验中只有两次显示出所有三个峰值。这些结果表明，激肽酶II进入肾小管液的主要部位靠近对氨基马尿酸峰值，并且63%的犬存在远端进入情况。
