Amory A M, Schubert C L
Biology Department, University of Natal, 4001, Durban, South Africa.
Oecologia. 1987 Sep;73(3):420-424. doi: 10.1007/BF00385259.
Protein determinations on protein-tannin complexes after protein isolation (gel filtration and trichloroacetic acid [TCA] precipitation) or phenolic extraction (polyvinyl pyrrolidone [PVP] and organic solvent precipitation) were unsuccessuful. Kjeldahl determinations of the amount of unprecipitated protein bovine serum albumin [BSA] showed a sigmoid relationship with increasing concentrations of tannins. A similar relationship was found for the reduced viscosity of BSA and plant protein, and the concentration of tannin. Non-linear regression and curve normalization allowed three variable (k , k and T ) to be defined for the quantification of the protein-tannin interaction/s. Such a treatment may be useful in studies of the role of tannins in plant-herbivore interactions.
在蛋白质分离（凝胶过滤和三氯乙酸 [TCA] 沉淀）或酚类提取（聚乙烯吡咯烷酮 [PVP] 和有机溶剂沉淀）后，对蛋白质 - 单宁复合物进行蛋白质测定未成功。凯氏定氮法测定未沉淀的蛋白质牛血清白蛋白 [BSA] 的量显示，其与单宁浓度增加呈 S 形关系。对于 BSA 和植物蛋白的比浓粘度以及单宁浓度，也发现了类似的关系。非线性回归和曲线归一化允许定义三个变量（k、k 和 T）来定量蛋白质 - 单宁相互作用。这种处理方法可能有助于研究单宁在植物 - 食草动物相互作用中的作用。
