Rainey D Y, Mitton J B, Monson R K
Department of Environmental, Population, and Organismic, University of Colorado, Biology Campus Box 334, 80309, Boulder, CO, USA.
Oecologia. 1987 Dec;74(3):335-338. doi: 10.1007/BF00378926.
In this study, we determined whether relationships existed between dark respiration and genotype at five enzyme polymorphisms in perennial ryegrass, Lolium perenne L. Positive correlations were found between Q of dark respiration and genotype at the phosphoglucomutase (PGM) and 6-phosphogluconate dehydrogenase (6PGD) loci. Plants doubly homozygous for the common allele at these loci were found to have Q values 20% higher than those for double heterozygotes. In plants that were heat stressed for five consecutive days, Q was found to be negatively correlated with apparent vigor after stressing. Individuals homozygous for PGM and 6PGD (with higher Q values) exhibited more apparent damage following the stress than heterozygous individuals. Both PGM and 6PGD occupy positions in metabolism with regulatory potential. Although caution must be used in assigning causal relationships, the results suggest that specific forms of these enzymes are directly related to, or are correlated with, the determinants of respiratory efficiency in L. perenne.
在本研究中，我们测定了多年生黑麦草（Lolium perenne L.）中黑暗呼吸与五个酶多态性位点的基因型之间是否存在关联。结果发现，黑暗呼吸的Q与磷酸葡萄糖变位酶（PGM）和6-磷酸葡萄糖酸脱氢酶（6PGD）位点的基因型之间存在正相关。在这些位点上对常见等位基因呈双纯合的植株，其Q值比双杂合子高20%。在连续五天受热胁迫的植株中，发现Q与胁迫后的表观活力呈负相关。PGM和6PGD纯合的个体（具有较高的Q值）在胁迫后比杂合个体表现出更明显的损伤。PGM和6PGD在具有调节潜力的代谢途径中均占据一定位置。尽管在确定因果关系时必须谨慎，但结果表明这些酶的特定形式与多年生黑麦草呼吸效率的决定因素直接相关或存在关联。
