爱泼斯坦-巴尔病毒gp85基因的鉴定
Identification of the Epstein-Barr virus gp85 gene.
作者信息
Heineman T, Gong M, Sample J, Kieff E
机构信息
Kovler Viral Oncology Laboratories, University of Chicago, Illinois 60637.
出版信息
J Virol. 1988 Apr;62(4):1101-7. doi: 10.1128/JVI.62.4.1101-1107.1988.
The Epstein-Barr virus glycoprotein gp85 has been mapped to the Epstein-Barr virus DNA open reading frame BXLF2 (R. Baer, A. Bankier, M. Biggin, P. Deininger, P. Farrell, T. Gibson, G. Hatfull, G. Hudson, S. Stachwell, C. Sequin, P. Tufnell, and B. Barrell, Nature [London] 310:207-211, 1984). A gp85-specific monoclonal antibody reacts with the BXLF2 in vitro transcription-translation product. The monoclonal antibody also precipitates an 85-kilodalton protein from rodent cells transfected with the BXLF2 open reading frame DNA. In these cells, gp85 localizes to the cytoplasm and nuclear rim rather than to the plasma membrane as in lymphocytes. Northern (RNA) blot hybridization and analysis of a cDNA clone containing BXLF2 indicate that gp85 is translated from an unspliced, late, 2.5-kilobase transcript. Similarities between the predicted amino acid sequences of gp85 and herpes simplex virus gH (D. McGeoch and A. Davison, Nucleic Acids Res. 14:4281-4292, 1986) are noted.
爱泼斯坦-巴尔病毒糖蛋白gp85已被定位到爱泼斯坦-巴尔病毒DNA开放阅读框BXLF2（R. 贝尔、A. 班基尔、M. 比金、P. 戴宁格、P. 法雷尔、T. 吉布森、G. 哈特富尔、G. 哈德森、S. 斯塔奇韦尔、C. 塞昆、P. 塔夫内尔和B. 巴雷尔，《自然》[伦敦]310:207 - 211，1984年）。一种gp85特异性单克隆抗体在体外转录-翻译产物中与BXLF2发生反应。该单克隆抗体还能从用BXLF2开放阅读框DNA转染的啮齿动物细胞中沉淀出一种85千道尔顿的蛋白质。在这些细胞中，gp85定位于细胞质和核边缘，而不像在淋巴细胞中那样定位于质膜。Northern（RNA）印迹杂交以及对包含BXLF2的cDNA克隆的分析表明，gp85是从一个未剪接的、晚期的2.5千碱基转录本翻译而来的。注意到gp85的预测氨基酸序列与单纯疱疹病毒gH（D. 麦吉奥克和A. 戴维森，《核酸研究》1:4281 - 4292，1986年）之间的相似性。
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