Shi Chung-Sheng, Li Jhy-Ming, Chin Chih-Chien, Kuo Yi-Hung, Lee Ying-Ray, Huang Yun-Ching
Graduate Institute of Clinical Medical Sciences, Chang Gung University, Taoyuan, Taiwan, R.O.C.
Division of Urology, Department of Surgery, Chang Gung Memorial Hospital, Chiayi, Taiwan, R.O.C.
Anticancer Res. 2017 Mar;37(3):1149-1159. doi: 10.21873/anticanres.11428.
BACKGROUND/AIM: Evodiamine, an indole alkaloid derived from Evodia rutaecarpa, exhibits pharmacological activities including vasodilatation, analgesia, anti-cardiovascular disease, anti-Alzheimer's disease, anti-inflammation, and anti-tumor activity.
This study analyzes the anti-tumor effects of evodiamine on cellular growth, tumorigenesis, cell cycle and apoptosis induction of human urothelial cell carcinoma (UCC) cells.
The present study showed that evodiamine significantly inhibited the proliferation of UCC cells in a dose- and time-dependent manner. Also, evodiamine suppressed the tumorigenesis of UCC cells in vitro. Moreover, evodiamine caused G/M cell-cycle arrest and induced caspase-dependent apoptosis in UCC cells. Finally, we demonstrated that evodiamine exhibits better cytotoxic than 5-fluorouracil, a clinical chemotherapeutic drug, for UCC cells.
Evodiamine induces growth inhibition, tumorigenesis suppression, cell-cycle arrest, and apoptosis induction in human UCC cells. Therefore, this agent displays a therapeutic potential for treating human UCC cells and is worthy for further investigation.
背景/目的：吴茱萸碱是一种从吴茱萸中提取的吲哚生物碱，具有多种药理活性，包括血管舒张、镇痛、抗心血管疾病、抗阿尔茨海默病、抗炎和抗肿瘤活性。
本研究分析了吴茱萸碱对人膀胱尿路上皮癌（UCC）细胞的细胞生长、肿瘤发生、细胞周期及凋亡诱导的抗肿瘤作用。
本研究表明，吴茱萸碱能以剂量和时间依赖性方式显著抑制UCC细胞的增殖。此外，吴茱萸碱在体外抑制了UCC细胞的肿瘤发生。而且，吴茱萸碱导致UCC细胞出现G/M期细胞周期阻滞并诱导了半胱天冬酶依赖性凋亡。最后，我们证明，对于UCC细胞，吴茱萸碱比临床化疗药物5-氟尿嘧啶具有更好的细胞毒性。
吴茱萸碱可诱导人UCC细胞生长抑制、肿瘤发生抑制、细胞周期阻滞及凋亡诱导。因此，该药物在治疗人UCC细胞方面显示出治疗潜力，值得进一步研究。
