在人神经母细胞瘤SK-N-MC细胞系中，β-肾上腺素能受体、血管活性肠肽（VIP）和神经肽Y（NPY）受体与腺苷酸环化酶功能偶联。
Beta-adrenoceptor, vasoactive intestinal polypeptide (VIP) and neuropeptide tyrosine (NPY) receptors functionally coupled to adenylate cyclase in the human neuroblastoma SK-N-MC cell line.
作者信息
Olasmaa M, Påhlman S, Terenius L
机构信息
Department of Pharmacology, Uppsala University, Sweden.
出版信息
Neurosci Lett. 1987 Dec 16;83(1-2):161-6. doi: 10.1016/0304-3940(87)90234-5.
Neurotransmitter receptor coupling to adenylate cyclase (AC) was studied in the cultured human neuroblastoma SK-N-MC cell line. Activation of beta-adrenoceptors with isoprenaline (ISO) or vasoactive intestinal polypeptide (VIP) receptors, increased AC activity in a dose-dependent manner. Preincubation with ISO and VIP induced a ligand specific, i.e. homologous type of desensitization of the respective receptor. Neuropeptide tyrosine (NPY) was able to inhibit ISO as well as VIP induced AC activity. The effect of NPY was totally abolished in cells pretreated with pertussis toxin to inactivate inhibitory G-proteins. Thus, SK-N-MC cells possess functionally coupled beta-adrenoceptors, VIP and NPY receptors, and may be used to study interactions between ligands and receptors which couple to the AC system.
在培养的人神经母细胞瘤SK-N-MC细胞系中研究了神经递质受体与腺苷酸环化酶（AC）的偶联。用异丙肾上腺素（ISO）激活β-肾上腺素能受体或血管活性肠肽（VIP）受体，可使AC活性呈剂量依赖性增加。用ISO和VIP预孵育可诱导各自受体的配体特异性脱敏，即同源脱敏。神经肽Y（NPY）能够抑制ISO以及VIP诱导的AC活性。在用百日咳毒素预处理以灭活抑制性G蛋白的细胞中，NPY的作用完全消失。因此，SK-N-MC细胞具有功能偶联的β-肾上腺素能受体、VIP和NPY受体，可用于研究与AC系统偶联的配体和受体之间的相互作用。
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