Okoyama S, Tago H, McGeer P L, Hersh L B, Kimura H
Department of Anatomy, School of Medicine, Kanazawa University, Japan.
Neurosci Lett. 1987 Dec 16;83(1-2):77-81. doi: 10.1016/0304-3940(87)90219-9.
Cholinergic neurons in the basal forebrain which project to both the hippocampus and olfactory bulb were identified in the rat by using twin fluorescent retrograde tracers combined with fluorescence immunohistochemistry for choline acetyltransferase (ChAT). The majority of neurons simultaneously labeled with the two tracers were also identified as being ChAT-positive. They were located at the border between the horizontal and vertical limbs of the diagonal band of Broca. Their numbers were very small compared with neurons singly labeled from each of the projection areas.
通过使用双荧光逆行示踪剂结合胆碱乙酰转移酶（ChAT）的荧光免疫组织化学方法，在大鼠中鉴定出投射到海马体和嗅球的基底前脑胆碱能神经元。大多数同时被两种示踪剂标记的神经元也被鉴定为ChAT阳性。它们位于布罗卡斜带水平支和垂直支的交界处。与从每个投射区域单独标记的神经元相比，它们的数量非常少。
