利用噬菌体展示产生的抗体检测食物过敏原

Detection of Food Allergens by Phage-Displayed Produced Antibodies.

作者信息

Madrid Raquel, de la Cruz Silvia, García Aina, Martín Rosario, González Isabel, García Teresa

机构信息

Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, Av. Puerta de Hierro a/n, 28040, Madrid, Spain.

出版信息

Methods Mol Biol. 2017;1592:109-128. doi: 10.1007/978-1-4939-6925-8_9.

DOI:10.1007/978-1-4939-6925-8_9

PMID:28315215

Abstract

Phage display is a powerful tool to produce recombinant antibodies against a given antigen without animal immunization. This technology employs libraries of recombinant bacteriophages that display billions of different functional antibody fragments on their surface. They are selected by panning in vitro against the target antigen in search for specific binders. In this chapter, we describe the selection of single chain variable fragment (scFv) antibodies to be used for detection of allergenic proteins from nuts in food products. The artificial libraries TomLinson I+J (MRC Laboratory of Molecular Biology and MRC Centre for Protein Engineering) were employed that resulted in successful phage-ELISA systems for detection of almond and walnut proteins in commercial food products.

摘要

噬菌体展示是一种无需动物免疫就能生产针对特定抗原的重组抗体的强大工具。该技术利用重组噬菌体文库，这些文库在其表面展示数十亿种不同的功能性抗体片段。通过体外淘选针对目标抗原的文库来筛选出特异性结合物。在本章中，我们描述了用于检测食品中坚果过敏原蛋白的单链可变片段（scFv）抗体的筛选。使用了人工构建的TomLinson I+J文库（分子生物学医学研究委员会实验室和蛋白质工程医学研究委员会中心），该文库成功构建了用于检测商业食品中杏仁和核桃蛋白的噬菌体酶联免疫吸附测定系统。