Quantitative characterization of enzyme adsorption and hydrolytic performance for ultrafine grinding pretreated corn stover.

Quantitative analysis of enzyme adsorption and hydrolysis were performed for sieve-based grinding corn stover (SGCS) and ultrafine grinding corn stover (UGCS) with different enzyme consumptions. The UGCS presented significantly higher enzyme adsorption quantity (5.15mg/g for UGCS, 1.33mg/g for SGCS), higher glucose yield (49.75% for UGCS, 28.75% for SGCS) under 20FPU/g and higher binding enzyme proportion (41.32% for UGCS, 10.64% for SGCS under 5FPU/g) which can be attributed to the more accessible microstructure properties. The relationship between enzyme adsorption and hydrolytic production was directly proportional for SGCS (GY=21.04×AQ+1.86 (R=0.95)) while was exponential for UGCS (GY=49.42×(1-e) (R=0.99)), indicating that overmuch enzyme consumption was not advisable for UGCS at economical aspect.