Key Laboratory of Forest Genetics and Biotechnology, Ministry of Education, Nanjing Forestry University, Nanjing 210037, China.
Bioresour Technol. 2011 Apr;102(7):4905-8. doi: 10.1016/j.biortech.2010.12.047. Epub 2010 Dec 22.
The feasibility of three-stage hydrolysis of steam-exploded corn stover at high-substrate concentration was investigated. When substrate concentration was 30% and enzyme loading was 15-30 FPU/g cellulose, three-stage (9+9+12 h) hydrolysis could reach a hydrolysis yield of 59.9-81.4% in 30 h. Compared with one-stage hydrolysis for 72 h, an increase of 34-37% in hydrolysis yield could be achieved. When steam-exploded corn stover was used as the substrate for enzyme synthesis and hydrolysis was conducted at a substrate concentration of 25% with an enzyme loading of 20 FPU/g cellulose, a hydrolysis yield of 85.1% was obtained, 19% higher than that the commercial cellulase could reach under the same conditions. The removal of end products was suggested to improve the adsorption of cellulase on the substrate and enhance the productivity of enzymatic hydrolysis.
研究了在高底物浓度下对蒸汽爆破玉米秸秆进行三阶段水解的可行性。当底物浓度为 30%，酶用量为 15-30 FPU/g 纤维素时，三阶段（9+9+12 h）水解在 30 h 内可达到 59.9-81.4%的水解产率。与 72 h 的一阶段水解相比，水解产率可提高 34-37%。当以蒸汽爆破玉米秸秆为酶合成的底物，并在 25%的底物浓度下，酶用量为 20 FPU/g 纤维素时，水解产率达到 85.1%，比相同条件下商业纤维素酶的产率高 19%。建议去除终产物以提高纤维素酶对底物的吸附，并提高酶解的生产力。
