Department of Chemistry, Washburn University, 1700 S College Avenue, Topeka, KS 66621, United States.
Department of Chemistry, Washburn University, 1700 S College Avenue, Topeka, KS 66621, United States.
Biochim Biophys Acta Proteins Proteom. 2017 Jun;1865(6):633-639. doi: 10.1016/j.bbapap.2017.03.005. Epub 2017 Mar 16.
Ragweed pollen is primarily responsible for the hay fever allergies of sufferers throughout the world. A proteome study of three ragweed plants (Ambrosia artemisiifolia, Ambrosia trifida, and Ambrosia psilostachya) was undertaken to document and compare their protein profiles. Proteins extracted from the pollen of the three plants were subjected to one dimensional electrophoresis followed by tandem liquid chromatography-mass spectroscopy. Peptide sequence mapping permitted discovery of proteins not previously reported for all three plants and 45% of the identified proteins were shared by all three of them. Application of stringent criteria revealed not only a majority of known allergens for short ragweed but also allergens not previously reported for the other two plants. Additionally, potentially allergy inducing enolases are reported for the three plants. These results suggest that all three ragweed plants could contribute to the allergy malady.
豚草花粉是全世界花粉过敏症患者的主要过敏原。为了记录和比较三种豚草（豚草、三裂豚草和豚草）的蛋白质图谱，进行了一项蛋白质组学研究。从三种植物的花粉中提取的蛋白质进行一维电泳，然后进行串联液相色谱-质谱分析。肽序列图谱分析发现了以前三种植物都没有报道过的蛋白质，其中 45%的鉴定蛋白质为三种植物所共有。应用严格的标准不仅揭示了短豚草的大多数已知过敏原，也揭示了其他两种植物以前没有报道过的过敏原。此外，还报告了三种植物中可能引起过敏的烯醇酶。这些结果表明，所有三种豚草植物都可能导致过敏症。
