Bordoni Laura, Napolioni Valerio, Marchegiani Francesca, Amadio Emilio, Gabbianelli Rosita
School of Advanced Studies, University of Camerino, Camerino, Italy.
J Nutrigenet Nutrigenomics. 2017;10(1-2):1-8. doi: 10.1159/000458154. Epub 2017 Mar 18.
The well-known insertion/deletion polymorphism (rs4646994) of the angiotensin-converting enzyme (ACE) gene has been previously associated with obesity, blood flow, muscular strength, and ACE enzyme activity. Despite the relevant role of ACE in homeostasis, few data are currently available on the relationship between rs4646994 and hydration status. Thus, we tested the association between the ACE Ins/Del polymorphism, body composition, and hydration status in a young Italian population.
A total of 306 healthy children and adolescents who regularly practice sports were recruited. Anthropometric, bioimpedentiometric parameters, and urine samples were collected, while ACE rs4646994 genotyping was performed on DNA from buccal swabs. General linear models were used for association testing.
The ACE Ins/Del polymorphism was associated with body composition. Ins/Ins individuals had higher phase angle (PhA) and body cellular mass index (BCMI) values. A significant influence of the ACE rs4646994 according to hydration status on body composition was also identified. In particular, Ins/Ins individuals displayed higher PhA and BCMI values only if norm-hydrated, while they showed values similar to Del carriers if dehydrated.
Our results confirm the relationship between the ACE Ins/Del polymorphism and body composition and suggest a role for hydration status in modulating this relationship. These interesting preliminary results warrant further investigation to disentangle the genetic role of ACE on hydration homeostasis.
血管紧张素转换酶（ACE）基因著名的插入/缺失多态性（rs4646994）先前已与肥胖、血流、肌肉力量及ACE酶活性相关。尽管ACE在体内平衡中具有重要作用，但目前关于rs4646994与水合状态之间关系的数据很少。因此，我们在意大利年轻人群中测试了ACE插入/缺失多态性、身体成分和水合状态之间的关联。
共招募了306名经常进行体育锻炼的健康儿童和青少年。收集人体测量、生物电阻抗参数及尿液样本，同时对口腔拭子DNA进行ACE rs4646994基因分型。使用一般线性模型进行关联测试。
ACE插入/缺失多态性与身体成分相关。插入/插入个体具有更高的相位角（PhA）和身体细胞质量指数（BCMI）值。还确定了根据水合状态，ACE rs4646994对身体成分有显著影响。特别是，插入/插入个体仅在正常水合时显示出较高的PhA和BCMI值，而在脱水时其值与缺失携带者相似。
我们的结果证实了ACE插入/缺失多态性与身体成分之间的关系，并表明水合状态在调节这种关系中起作用。这些有趣的初步结果值得进一步研究，以阐明ACE在水合稳态中的遗传作用。
