Ostadhadi Sattar, Haddadi Nazgol-Sadat, Foroutan Arash, Azimi Ehsan, Elmariah Sarina, Dehpour Ahmad-Reza
Brain and Spinal Cord Injury Research Center, Neurosciences Institute, Tehran University of Medical Sciences, Tehran, Iran.
Experimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Clin Exp Pharmacol Physiol. 2017 Jun;44(6):680-685. doi: 10.1111/1440-1681.12752.
Cholestatic itch can be severe and significantly impair the quality of life of patients. The serotonin system is implicated in cholestatic itch; however, the pruritogenic properties of serotonin have not been evaluated in cholestatic mice. Here, we investigated the serotonin-induced itch in cholestatic mice which was induced by bile duct ligation (BDL). Serotonin, sertraline or saline were administered intradermally to the rostral back area in BDL and sham operated (SHAM) mice, and the scratching behaviour was videotaped for 1 hour. Bile duct ligated mice had significantly increased scratching responses to saline injection on the seventh day after surgery. Additionally, serotonin or sertraline significantly induced scratching behaviour in BDL mice compared to saline at day 7 after surgery, while it did not induce itch at day 5. The scratching behaviour induced by serotonin or sertraline was significantly less in BDL mice compared to SHAM mice. Likewise, the locomotor activity of BDL or SHAM mice was not significantly different from unoperated (UNOP) mice on the fifth and seventh day, suggesting that the scratching behaviour was not affected by motor dysfunctions. Our data suggest that despite the potentiation of evoked itch, a resistance to serotonin-induced itch is developed in cholestatic mice.
胆汁淤积性瘙痒可能很严重，并会显著损害患者的生活质量。血清素系统与胆汁淤积性瘙痒有关；然而，血清素的致痒特性尚未在胆汁淤积小鼠中得到评估。在此，我们研究了胆管结扎（BDL）诱导的胆汁淤积小鼠中血清素诱导的瘙痒。将血清素、舍曲林或生理盐水皮内注射到BDL小鼠和假手术（SHAM）小鼠的背部头侧区域，并对抓挠行为进行1小时的录像。胆管结扎小鼠在术后第7天对生理盐水注射的抓挠反应显著增加。此外，与生理盐水相比，血清素或舍曲林在术后第7天显著诱导BDL小鼠的抓挠行为，而在第5天未诱导瘙痒。与SHAM小鼠相比，BDL小鼠中血清素或舍曲林诱导的抓挠行为明显较少。同样，BDL或SHAM小鼠在第5天和第7天的运动活动与未手术（UNOP）小鼠没有显著差异，这表明抓挠行为不受运动功能障碍的影响。我们的数据表明，尽管诱发的瘙痒增强，但胆汁淤积小鼠对血清素诱导的瘙痒产生了抗性。
