Departments of Molecular Biology and Immunology, Laboratorios Clínicos de Puebla, Puebla, México.
School of Medicine, Universidad Popular Autónoma del Estado de Puebla, Puebla, México.
Cytometry B Clin Cytom. 2018 Jan;94(1):172-181. doi: 10.1002/cyto.b.21523. Epub 2017 Mar 29.
The aim of this work was to simultaneously use multiplex ligation-dependent probe amplification (MLPA) assay and flow cytometric DNA ploidy analysis (FPA) to detect aneuploidy in patients with newly diagnosed acute leukemia.
MLPA assay and propidium iodide FPA were used to test samples from 53 consecutive patients with newly diagnosed acute leukemia referred to our laboratory for immunophenotyping. Results were compared by nonparametric statistics.
The combined use of both methods significantly increased the rate of detection of aneuploidy as compared to that obtained by each method alone. The limitations of one method are somehow countervailed by the other and vice versa.
MPLA and FPA yield different yet complementary information concerning aneuploidy in acute leukemia. The simultaneous use of both methods might be recommended in the clinical setting. © 2017 International Clinical Cytometry Society.
本研究旨在通过多重连接依赖性探针扩增（MLPA）检测和流式细胞术 DNA 倍体分析（FPA）联合检测新诊断急性白血病患者的非整倍体。
应用 MLPA 检测和碘化丙啶 FPA 对 53 例新诊断急性白血病患者进行免疫表型分析。采用非参数统计方法比较结果。
与单一方法相比，两种方法联合使用显著提高了非整倍体的检测率。一种方法的局限性可以通过另一种方法得到弥补，反之亦然。
MLPA 和 FPA 提供了有关急性白血病非整倍体的不同但互补的信息。在临床环境中推荐同时使用这两种方法。© 2017 国际临床细胞遗传学学会。
