Li L H, Xu M P, Gan L M, Li Y, Liang Y L, Li W T, Qin E Y, Gan J H, Liu G N
Department of Respiratory, Second Affiliated Hospital of Guangxi Medical University, Nanning 530007, China.
Zhonghua Yi Xue Za Zhi. 2017 Mar 14;97(10):777-781. doi: 10.3760/cma.j.issn.0376-2491.2017.10.012.
To investigate the effect of low dose erythromycin on the proliferation of granulation tissue after tracheal injury. Forty-two rabbits were randomly divided into 7 groups (=6 each), group A (saline control group), group B (penicillin group), group C (low dose erythromycin group), group D (low dose erythromycin and penicillin group), group E (budesonide group), group F (low dose erythromycin and budesonide group), group G (low dose erythromycin, penicillin and budesonide group). All rabbits received tracheotomy, and the tracheal mucosa was scraped with a nylon brush 20 times for tracheal stenosis model. Rabbits were treated with corresponding drugs from a week before operation to 9 days after operation. The serum concentrations of transforming growth factor - beta 1 (TGF-β(1)), vascular endothelial growth factor (VEGF), interleukin (IL) -6, IL-8 were determined and the tracheal specimens were harvested for measuring degree of stenosis on the 10th day after operation. Serum concentrations of TGF-β(1) in group A, B, C, D, E, F and G were (17.6±1.3), (18.2±3.1), (13.0±1.1), (14.0±1.0), (21.0±6.1), (13.6± 3.5), (8.2±1.3) ng/L; VEGF were (88.1±4.1), (85.8±4.3), (58.1±6.3), (56.5±2.4), (87.8±2.8), (57.0±3.7), (34.3±6.7) ng/L; IL-6 were (67.8±4.0), (66.1±3.5), (54.1±4.8), (52.1±3.2), (64.6±4.9), (49.4±4.2), (35.9±3.7) ng/L; IL-8 were (112.8±5.2), (116.6±4.1), (88.0±6.2), (85.5±3.5), (114.4±4.6), (82.6±3.8), (55.9±6.0) ng/L, respectively. The serum concentrations of TGF-β(1), VEGF, IL-6 and IL-8 in group C, D, F and G were significantly lower than those in group A, B and E (all <0.05). Compared with the other groups, the serum concentrations in group G were the lowest (all <0.05). All 42 rabbits had tracheal stenosis with different degrees of proliferation of granulation tissue. The degree of tracheal stenosis in Group A, B, C, D, E, F and G were (53.3±4.4)%, (48.2±5.0)%, (24.3±4.4)%, (29.5±3.2)%, (47.8±6.5)%, (27.9±3.1)%, (15.6±2.0)%, respectively. The degree of tracheal stenosis in group C, D, F and G was significantly lower than that in group A, B and E, which had statistical differences (all <0.05). Compared with the other groups, the degree of tracheal stenosis in group G was the lowest (all <0.05). Low dose of erythromycin can effectively inhibit the proliferation of granulation tissue after tracheal injury in rabbits. And it has better effectiveness when combined with other antibiotics and hormone.
探讨低剂量红霉素对气管损伤后肉芽组织增殖的影响。将42只家兔随机分为7组（每组6只），A组（生理盐水对照组）、B组（青霉素组）、C组（低剂量红霉素组）、D组（低剂量红霉素与青霉素组）、E组（布地奈德组）、F组（低剂量红霉素与布地奈德组）、G组（低剂量红霉素、青霉素与布地奈德组）。所有家兔均行气管切开术，并用尼龙刷刮擦气管黏膜20次以建立气管狭窄模型。家兔于术前1周开始至术后9天给予相应药物治疗。于术后第10天测定血清转化生长因子-β1（TGF-β1）、血管内皮生长因子（VEGF）、白细胞介素（IL）-6、IL-8浓度，并取气管标本测量狭窄程度。A组、B组、C组、D组、E组、F组和G组血清TGF-β1浓度分别为（17.6±1.3）、（18.2±3.1）、（13.0±1.1）、（14.0±1.0）、（21.0±6.1）、（13.6±3.5）、（8.2±1.3）ng/L；VEGF分别为（88.1±4.1）、（85.8±4.3）、（58.1±6.3）、（56.5±2.4）、（87.8±2.8）、（57.0±3.7）、（34.3±6.7）ng/L；IL-6分别为（67.8±4.0）、（66.1±3.5）、（54.1±4.8）、（52.1±3.2）、（64.6±'4.9）、（49.4±4.2）、（35.9±3.7）ng/L；IL-8分别为（112.8±5.2）、（116.6±4.1）、（88.0±6.2）、（85.5±3.5）、（114.4±4.6）、（82.6±3.8）、（55.9±6.0）ng/L。C组、D组、F组和G组血清TGF-β1、VEGF、IL-6和IL-8浓度均显著低于A组、B组和E组（均P<0.05）。与其他组相比，G组血清浓度最低（均P<0.05）。42只家兔均出现气管狭窄，伴有不同程度的肉芽组织增殖。A组、B组、C组、D组、E组、F组和G组气管狭窄程度分别为（53.3±4.4）%、（48.2±5.0）%、（24.3±4.4）%、（29.5±3.2）%、（47.8±6.5）%、（27.9±3.1）%、（15.6±2.0）%。C组、D组、F组和G组气管狭窄程度显著低于A组、B组和E组，差异有统计学意义（均P<0.05）。与其他组相比，G组气管狭窄程度最低（均P<0.05）。低剂量红霉素可有效抑制家兔气管损伤后肉芽组织的增殖。与其他抗生素及激素联合应用时效果更佳。
