蜱虫（蜱螨亚纲：硬蜱科）抗氧化反应和酯酶谱的性别差异
Sexual alteration in antioxidant response and esterase profile in (Acari: Ixodidae) ticks.
作者信息
Ghosh Mayukh, Sangwan Nirmal, Sangwan Arun K, Kumar Rajesh, Gaur Ruchi Singh
机构信息
Department of Veterinary Physiology and Biochemistry, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana 125004 India.
Department of Veterinary Parasitology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana 125004 India.
出版信息
J Parasit Dis. 2017 Mar;41(1):106-111. doi: 10.1007/s12639-016-0758-5. Epub 2016 Apr 4.
The objective of the study was to reveal physiological link between sex specific engorgement pattern and evading mechanism of ticks against oxidative stress as well as acaricides. Quantitative determination of nitric oxide radical scavenging, superoxide dismutase activity and reduced glutathione (GSH) concentrations in salivary gland and gut extracts of male and female (Acari: Ixodidae) ticks established significant variation in antioxidant responses between two sexes of the ticks. Higher activity of these antioxidants and GSH depletion in females clearly indicate stronger antioxidant defense in female ticks which is to combat host mediated oxidative assault during feeding for greater engorgement and reproductive stress. The females are also better equipped with the mechanism of acaricide resistance as evidenced by higher expression of esterases than males in unfed whole tick extracts in current study.
该研究的目的是揭示特定性别充血模式与蜱虫抵御氧化应激及杀螨剂的逃避机制之间的生理联系。对雌雄（蜱螨目：硬蜱科）蜱虫唾液腺和肠道提取物中的一氧化氮自由基清除能力、超氧化物歧化酶活性和还原型谷胱甘肽（GSH）浓度进行定量测定，结果表明蜱虫两性之间的抗氧化反应存在显著差异。雌性蜱虫中这些抗氧化剂的活性较高以及GSH消耗明显，清楚地表明雌性蜱虫具有更强的抗氧化防御能力，这是为了在进食期间对抗宿主介导的氧化攻击，以实现更大程度的充血和生殖应激。本研究还表明，未进食的全蜱提取物中，雌性蜱虫酯酶表达高于雄性，这证明雌性蜱虫对杀螨剂的抗性机制也更强。
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