Liu Youxun, Wang Yaokun, Yan Mingyang, Huang Juan
School of Basic Medical Sciences, Xinxiang Medical University, Jinsui Avenue 601, Xinxiang, Henan 453003, China; Henan Collaborative Innovation Center of Molecular Diagnostics and Laboratory Medicine, Jinsui Avenue 601, Xinxiang, Henan 453003, China.
School of Basic Medical Sciences, Xinxiang Medical University, Jinsui Avenue 601, Xinxiang, Henan 453003, China.
Biomed Res Int. 2017;2017:7309481. doi: 10.1155/2017/7309481. Epub 2017 Feb 21.
Hierarchical copper shells anchored on magnetic nanoparticles were designed and fabricated to selectively deplete hemoglobin from human blood by immobilized metal affinity chromatography. Briefly, CoFeO nanoparticles coated with polyacrylic acid were first synthesized by a one-pot solvothermal method. Hierarchical copper shells were then deposited by immobilizing Cu on nanoparticles and subsequently by reducing between the solid CoFeO@COOH and copper solution with NaBH. The resulting nanoparticles were characterized by scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectrometry, X-ray photoelectron spectroscopy, and vibrating sample magnetometry. The particles were also tested against purified bovine hemoglobin over a range of pH, contact time, and initial protein concentration. Hemoglobin adsorption followed pseudo-second-order kinetics and reached equilibrium in 90 min. Isothermal data also fit the Langmuir model well, with calculated maximum adsorption capacity 666 mg g. Due to the high density of Cu on the shell, the nanoparticles efficiently and selectively deplete hemoglobin from human blood. Taken together, the results demonstrate that the particles with hierarchical copper shells effectively remove abundant, histidine-rich proteins, such as hemoglobin from human blood, and thereby minimize interference in diagnostic and other assays.
设计并制备了锚定在磁性纳米颗粒上的分级铜壳，通过固定化金属亲和色谱法从人血中选择性去除血红蛋白。简要地说，首先通过一锅溶剂热法合成涂覆有聚丙烯酸的CoFeO纳米颗粒。然后通过将Cu固定在纳米颗粒上，随后用NaBH在固体CoFeO@COOH和铜溶液之间进行还原，来沉积分级铜壳。通过扫描电子显微镜、透射电子显微镜、傅里叶变换红外光谱、X射线光电子能谱和振动样品磁强计对所得纳米颗粒进行表征。还在一系列pH值、接触时间和初始蛋白质浓度下对这些颗粒针对纯化的牛血红蛋白进行了测试。血红蛋白吸附遵循准二级动力学，90分钟内达到平衡。等温数据也很好地拟合了朗缪尔模型，计算出的最大吸附容量为666 mg/g。由于壳上Cu的高密度，这些纳米颗粒能有效地从人血中选择性去除血红蛋白。综上所述，结果表明具有分级铜壳的颗粒能有效去除人血中丰富的、富含组氨酸的蛋白质，如血红蛋白，从而将对诊断和其他检测的干扰降至最低。
