Blanco R, Saenz de Tejada I, Goldstein I, Krane R J, Wotiz H H, Cohen R A
Department of Urology, Boston University Medical Center, Massachusetts 02118.
Am J Physiol. 1988 Mar;254(3 Pt 2):H468-72. doi: 10.1152/ajpheart.1988.254.3.H468.
Physiological and histochemical evidence indicates that cholinergic nerves may participate in mediating penile erection. Acetylcholine synthesis and release was studied in isolated human corporal tissue. Human corpus cavernosum incubated with [3H )choline accumulated [3H]choline and synthesized [3H]acetylcholine in a concentration-dependent manner. [3H]Acetylcholine accumulation by the tissue was inhibited by hemicholinium-3, a specific antagonist of the high-affinity choline transport in cholinergic nerves. Transmural electrical field stimulation caused release of [3H]acetylcholine which was significantly diminished by inhibiting neurotransmission with calcium-free physiological salt solution or tetrodotoxin. These observations provide biochemical and physiological evidence for the existence of cholinergic innervation in human corpus cavernosum.
生理学和组织化学证据表明，胆碱能神经可能参与介导阴茎勃起。对分离出的人体海绵体组织中的乙酰胆碱合成与释放进行了研究。用[3H]胆碱孵育的人体海绵体以浓度依赖的方式积累[3H]胆碱并合成[3H]乙酰胆碱。该组织对[3H]乙酰胆碱的积累受到3-甲基胆碱的抑制，3-甲基胆碱是胆碱能神经中高亲和力胆碱转运的特异性拮抗剂。经壁电场刺激导致[3H]乙酰胆碱释放，而用无钙生理盐溶液或河豚毒素抑制神经传递可显著减少这种释放。这些观察结果为人体海绵体中胆碱能神经支配的存在提供了生化和生理学证据。
