Wei Lili, Zhao Xuemei, Wang Ruanlin, Fu Yuejun, Chai Baofeng, Liang Aihua
a Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology , Shanxi University , Taiyuan , China.
Biosci Biotechnol Biochem. 2017 Jul;81(7):1327-1334. doi: 10.1080/09168451.2017.1301804. Epub 2017 Mar 20.
Analysis of transcriptome revealed that a membrane occupation and recognition nexus (MORN) repeat protein-encoding gene of Euplotes octocarinatus (Eo-morn-9-31) was a candidate for programmed +1 ribosomal frameshifting (+1 PRF). In this study, a dual-luciferase assay was performed to detect its expression. The result showed that the MORN repeat protein (Eo-MORN-9-31) could be produced by the +1 PRF event during the process of translation in yeast and the frameshifting efficiency was about 4-5%. We further confirmed its reality by western blot and mass spectrometry. This study provided experimental evidence indicating that the expression of the Eo-MORN-9-31 of E. octocarinatus required the +1 PRF.
转录组分析表明，八肋游仆虫的一个膜占据与识别连接（MORN）重复蛋白编码基因（Eo-morn-9-31）是程序性 +1 核糖体移码（+1 PRF）的一个候选基因。在本研究中，进行了双荧光素酶测定以检测其表达。结果表明，MORN 重复蛋白（Eo-MORN-9-31）可在酵母翻译过程中由 +1 PRF 事件产生，且移码效率约为 4 - 5%。我们通过蛋白质免疫印迹和质谱进一步证实了其真实性。本研究提供了实验证据，表明八肋游仆虫 Eo-MORN-9-31 的表达需要 +1 PRF。
