Zheng Mingming, Wang Shi, Xiang Xia, Shi Jie, Huang Juan, Deng Qianchun, Huang Fenghong, Xiao Junyong
Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Hubei Key Laboratory of Lipid Chemistry and Nutrition, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China.
Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Hubei Key Laboratory of Lipid Chemistry and Nutrition, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China; Functional Oil Laboratory Associated by Oil Crops Research Institute, Chinese Academy of Agricultural Sciences and Infinite (China) Co. LTD, Guangzhou 51000, China.
Food Chem. 2017 Aug 1;228:476-483. doi: 10.1016/j.foodchem.2017.01.129. Epub 2017 Jan 30.
In this study, Candida lipolytica lipase (CLL) was immobilized on magnetic multi-walled carbon nanotubes (mMWCNTs) via hydrophobic and cation-exchange interaction. The resultant immobilized CLL showed much better thermal stability, biocatalyst activity and easier recycling than the free form. A method for efficient enzymatic acidolysis of tripalmitin (PPP) with oleic acid (OA), to produce OPO-rich TAGs, was developed, using the immobilized CLL as the biocatalyst. Under optimized conditions (2% water, 20mg/ml enzyme, 1:6 PPP/OA, 50°C, 2h), the content of OPO in the final product reached 46.5%. CLL@mMWCNTs had a better activity and manipulative stability than commercial lipases. More importantly, the feasibility of CLL@mMWCNTs was also validated in the practical production of OPO-rich TAGs, using lard and restructured palm oil as the raw material. These results suggest that CLL@mMWCNTs is a promising biocatalyst for the OPO-rich TAGs production and will be helpful for the infant formula industry.
在本研究中，解脂耶氏酵母脂肪酶（CLL）通过疏水作用和阳离子交换相互作用固定在磁性多壁碳纳米管（mMWCNTs）上。所得固定化CLL与游离形式相比，具有更好的热稳定性、生物催化活性和更易回收利用的特点。利用固定化CLL作为生物催化剂，开发了一种用油酸（OA）对三棕榈酸甘油酯（PPP）进行高效酶促酸解以生产富含1,3-二油酸-2-棕榈酸甘油三酯（OPO）的甘油三酯（TAGs）的方法。在优化条件（2%水、20mg/ml酶、1:6的PPP/OA、50°C、2小时）下，最终产物中OPO的含量达到46.5%。CLL@mMWCNTs比商业脂肪酶具有更好的活性和操作稳定性。更重要的是，以猪油和重组棕榈油为原料，在富含OPO的TAGs实际生产中也验证了CLL@mMWCNTs的可行性。这些结果表明，CLL@mMWCNTs是用于生产富含OPO的TAGs的一种有前景的生物催化剂，将对婴儿配方奶粉行业有所帮助。
