Ramírez-Ramírez Edgar, Torres-Ramírez Armando, Alquicira-Mireles Jesús, Cañavera-Constantino Abraham, Segura-Medina Patricia, Montaño-Ramírez Martha, Ramos-Abraham Carlos, Vargas Mario H, Arreola-Ramírez José Luis
a Departamento de Investigación en Hiperreactividad Bronquial , Instituto Nacional de Enfermedades Respiratorias , Ciudad de México , México.
b Facultad de Medicina , Universidad Autónoma de Coahuila , Torreón , Coahuila , México.
Exp Lung Res. 2017 Mar;43(2):57-65. doi: 10.1080/01902148.2017.1294632. Epub 2017 Mar 20.
Long-term exposure to cigarette smoke generates chronic obstructive pulmonary disease (COPD) in guinea pigs, but a comprehensive evaluation of changes in lung function, as assessed by barometric whole body plethysmography (WBP), is lacking.
Female guinea pigs were exposed to the smoke of 20 cigarettes/day, 5 days/week, during 10 weeks (COPD group, n = 8), and were compared with unexposed female guinea pigs of the same age (control group, n = 8). WBP was performed in both groups, followed by lung histology.
At the end of the exposure period, guinea pigs in the COPD group had higher respiratory frequency, while duty cycle (Ti/Ttot) was unaffected. There was a trend toward minute ventilation (MV) and expiratory flow at the mid-tidal volume (EF50) to be higher in the COPD group. Enhanced pause (Penh) was lower, while time of braking (TB) and time to PEF relative to Te (Rpef) were higher in the COPD group. All guinea pigs exposed to tobacco smoke developed emphysematous lesions in their lungs and gained less body weight than controls.
In this COPD model, exposure to cigarette smoke produced changes in WBP characterized by a shallow breathing pattern with decreased Penh and a trend toward increasing EF50 (probably due to decreased elastic recoil), increased TB (suggesting dynamic laryngeal narrowing), and a trend of increasing MV (probably due to a higher metabolic rate). Many of these functional changes resemble those observed in patients with COPD and corroborate the suitability of this guinea pig model for the study of COPD.
长期暴露于香烟烟雾会使豚鼠患上慢性阻塞性肺疾病（COPD），但缺乏通过气压式全身体积描记法（WBP）对肺功能变化进行的全面评估。
雌性豚鼠每周5天，每天暴露于20支香烟的烟雾中，持续10周（COPD组，n = 8），并与相同年龄的未暴露雌性豚鼠（对照组，n = 8）进行比较。两组均进行WBP检查，随后进行肺组织学检查。
在暴露期结束时，COPD组豚鼠的呼吸频率较高，而占空比（Ti/Ttot）未受影响。COPD组的分钟通气量（MV）和潮气量中期呼气流量（EF50）有升高趋势。增强暂停（Penh）较低，而COPD组的制动时间（TB）和相对于呼气时间（Te）的呼气峰值流速时间（Rpef）较高。所有暴露于烟草烟雾的豚鼠肺部均出现肺气肿病变，体重增加少于对照组。
在这个COPD模型中，暴露于香烟烟雾导致WBP发生变化，其特征为呼吸模式变浅，Penh降低，EF50有增加趋势（可能由于弹性回缩降低），TB增加（提示动态喉部狭窄），MV有增加趋势（可能由于代谢率较高）。其中许多功能变化与COPD患者中观察到的变化相似，证实了这个豚鼠模型适用于COPD研究。
