Andre P, Capo C, Mege J L, Benoliel A M, Bongrand P
Laboratoire d'Immunologie, Hôpital de Sainte Marguerite, Marseille, France.
Biochem Biophys Res Commun. 1988 Mar 15;151(2):641-8. doi: 10.1016/s0006-291x(88)80329-2.
We found that rat bone marrow-derived macrophages responded to opsonized zymosan by releasing superoxide anion. However, these cells were defective in the response to the potent oxidative burst activator phorbol myristate acetate (PMA). This result was observed whatever the concentration of agonist used and with different concentrations of cells. Since it is strongly suspected that protein kinase C (PKC) is involved in the transductional pathway induced by PMA in numerous cell types, and particularly in phagocytes, we studied PKC and we observed that it was functional in rat bone marrow-derived macrophages, but only present at a low level. Thus, we suggest that our results are consistent with the possibility that zymosan-induced respiratory burst may be independent of PKC and that these cells may not possess the minimal level of PKC required for responding to PMA.
我们发现，大鼠骨髓来源的巨噬细胞通过释放超氧阴离子对调理酵母聚糖作出反应。然而，这些细胞对强效氧化爆发激活剂佛波酯（PMA）的反应存在缺陷。无论使用何种浓度的激动剂以及不同浓度的细胞，均观察到这一结果。由于人们强烈怀疑蛋白激酶C（PKC）参与了多种细胞类型中由PMA诱导的转导途径，尤其是在吞噬细胞中，因此我们对PKC进行了研究，发现它在大鼠骨髓来源的巨噬细胞中有功能，但仅以低水平存在。因此，我们认为我们的结果与以下可能性一致：酵母聚糖诱导的呼吸爆发可能独立于PKC，并且这些细胞可能不具备对PMA作出反应所需的最低水平的PKC。
