多聚（A）尾阻止 RDR6 将自身 mRNA 转化为基因沉默的底物。
The poly(A) tail blocks RDR6 from converting self mRNAs into substrates for gene silencing.
机构信息
Institute of Molecular and Cellular Biosciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-0032, Japan.
Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-0032, Japan.
出版信息
Nat Plants. 2017 Mar 20;3:17036. doi: 10.1038/nplants.2017.36.
It remains unclear how post-transcriptional gene silencing (PTGS) in plants discriminates aberrant RNAs from canonical messenger RNAs (mRNAs). The key step of plant PTGS is the conversion of aberrant RNAs into double-stranded RNAs by RNA-DEPENDENT RNA POLYMERASE6 (RDR6). Here, we show that RDR6 itself selects aberrant poly(A)-less mRNAs over canonical polyadenylated mRNAs as templates at the initiation step of complementary strand synthesis. This mechanism can be viewed as an innate safeguard against 'self-attack' by PTGS.
植物中转录后基因沉默（PTGS）如何区分异常 RNA 和规范的信使 RNA（mRNA）尚不清楚。植物 PTGS 的关键步骤是由 RNA 依赖性 RNA 聚合酶 6（RDR6）将异常 RNA 转化为双链 RNA。在这里，我们表明，在互补链合成的起始步骤中，RDR6 本身会选择异常的无 poly(A) 的 mRNA 而不是规范的多聚腺苷酸化的 mRNA 作为模板。这种机制可以被视为 PTGS 自我攻击的先天保护机制。
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