Samuels H H, Klein D, Stanley F, Casanova J
J Biol Chem. 1978 Sep 10;253(17):5895-8.
The induction of growth hormone synthesis and mRNA by thyroid hormone in cultured GH1 cells is mediated by the thyroid hormone nuclear receptor. In addition, the regulation of the growth hormone response by glucocorticoid is highly dependent on the action of thyroid hormone. To clarify whether thyroid hormone has a general influence on glucocorticoid action in GH1 cells, the glucocorticoid induction of growth hormone and glutamine synthetase was simultaneously examined. In contrast to the growth hormone response, the induction of glutamine synthetase by glucocorticoid was not influenced by thyroid hormone. Both responses appear to be modulated by the glucocorticoid receptor, and thyroid hormone had no influence on nuclear-associated glucocorticoid receptor levels. These results suggest that the thyroid hormone control of glucocorticoid induction of growth hormone may be a selective process, and the nuclear associated receptors for both thyroid and glucocorticoid hormones interrelate to control the growth hormone response.
甲状腺激素在培养的GH1细胞中对生长激素合成及信使核糖核酸的诱导作用是由甲状腺激素核受体介导的。此外，糖皮质激素对生长激素反应的调节高度依赖于甲状腺激素的作用。为了阐明甲状腺激素是否对GH1细胞中糖皮质激素的作用有普遍影响，同时检测了糖皮质激素对生长激素和谷氨酰胺合成酶的诱导作用。与生长激素反应不同，糖皮质激素对谷氨酰胺合成酶的诱导作用不受甲状腺激素影响。两种反应似乎均由糖皮质激素受体调节，且甲状腺激素对与细胞核相关的糖皮质激素受体水平没有影响。这些结果表明，甲状腺激素对糖皮质激素诱导生长激素的控制可能是一个选择性过程，甲状腺激素和糖皮质激素的细胞核相关受体相互关联以控制生长激素反应。
