骆驼蓬乙醇提取物及II型毒素抗毒素系统在生物膜形成中的作用
The Role of Peganum harmala Ethanolic Extract and Type II Toxin Antitoxin System in Biofilm Formation.
作者信息
Valizadeh Nasrin, Valian Firuzeh, Sadeghifard Nourkhoda, Karami Shahriar, Pakzad Iraj, Kazemian Hossein, Ghafourian Sobhan
机构信息
Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran.
Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, University Putra Malaysia, Malaysia.
出版信息
Drug Res (Stuttg). 2017 Jul;67(7):385-387. doi: 10.1055/s-0043-102060. Epub 2017 Mar 20.
Toxin antitoxin system is a regulatory system that antitoxin inhibits the toxin. We aimed to determine the role of TA loci in biofilm formation in K. pneumoniae clinical and environmental isolates; also inhibition of biofilm formation by Peganum harmala. So, 40 K. pneumoniae clinical and environmental isolates were subjected for PCR to determine the frequency of mazEF, relEB, and mqsRA TA loci. Biofilm formation assay subjected for all isolates. Then, P. harmala was tested against positive biofilm formation strains. Our results demonstrated that relBE TA loci were dominant TA loci; whereas mqsRA TA loci were negative in all isolates. The most environmental isolates showed weak and no biofilm formation while strong and moderate biofilm formation observed in clinical isolates. Biofilm formations by K. pneumoniae in 9 ug/ml concentration were inhibited by P. harmala. In vivo study suggested to be performed to introduce Peganum harmala as anti-biofilm formation in K. pneumoniae.
毒素-抗毒素系统是一种抗毒素抑制毒素的调节系统。我们旨在确定毒素-抗毒素基因座在肺炎克雷伯菌临床和环境分离株生物膜形成中的作用；同时确定骆驼蓬对生物膜形成的抑制作用。因此，对40株肺炎克雷伯菌临床和环境分离株进行PCR检测，以确定mazEF、relEB和mqsRA毒素-抗毒素基因座的频率。对所有分离株进行生物膜形成测定。然后，对骆驼蓬针对生物膜形成阳性菌株进行测试。我们的结果表明，relBE毒素-抗毒素基因座是主要的毒素-抗毒素基因座；而mqsRA毒素-抗毒素基因座在所有分离株中均为阴性。大多数环境分离株显示出较弱或无生物膜形成，而临床分离株中观察到较强和中等程度的生物膜形成。9微克/毫升浓度的骆驼蓬可抑制肺炎克雷伯菌的生物膜形成。建议进行体内研究，以引入骆驼蓬作为肺炎克雷伯菌生物膜形成的抑制剂。
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