视紫红质发色团与膜脂缺乏相互作用。一项使用14N:15N对的电子-电子双共振研究。
Lack of interaction of rhodopsin chromophore with membrane lipids. An electron-electron double resonance study using 14N:15N pairs.
作者信息
Renk G E, Crouch R K, Feix J B
机构信息
Department of Biochemistry, Medical University of South Carolina, Charleston 29425-2236.
出版信息
Biophys J. 1988 Mar;53(3):361-5. doi: 10.1016/S0006-3495(88)83113-8.
Electron-electron double resonance (ELDOR) has been applied to the study of specific interactions of 15N-spin-labeled stearic acid with the retinal chromophore of a rhodopsin analogue containing a 14N spin-labeled retinal. Both the 5 and 16 spin-labeled stearic acids were incorporated into the lipid bilayer of rod outer segment membranes containing the spin-labeled pigment. No interaction between the 15N and 14N spin-labels was observed in rhodopsin or the metarhodopsin II state with either of these labeled stearic acids. Therefore in this system the ring portion of the chromophore must be highly sequestered from the phospholipid bilayer in both the rhodopsin and metarhodopsin II forms.
电子-电子双共振（ELDOR）已被用于研究15N自旋标记的硬脂酸与含有14N自旋标记视黄醛的视紫红质类似物的视黄醛发色团之间的特定相互作用。5位和16位自旋标记的硬脂酸都被掺入到含有自旋标记色素的视杆外段膜的脂质双层中。在视紫红质或变视紫红质II状态下，使用这两种标记的硬脂酸均未观察到15N和14N自旋标记之间的相互作用。因此，在该系统中，视紫红质和变视紫红质II形式的发色团环部分必须与磷脂双层高度隔离。
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