生物膜中脂质不对称性形成的跨膜偶联机制。应用于光感受器盘膜。
Transbilayer coupling mechanism for the formation of lipid asymmetry in biological membranes. Application to the photoreceptor disc membrane.
作者信息
Hubbell W L
机构信息
Jules Stein Eye Institute, University of California, Los Angeles 90024.
出版信息
Biophys J. 1990 Jan;57(1):99-108. doi: 10.1016/S0006-3495(90)82510-8.
Abstract
An equilibrium transmembrane asymmetry in charged lipids is shown to arise as a result of oriented, bipolar proteins in the membrane. The basic interaction giving rise to the asymmetry is between a lipid molecule and a transbilayer potential generated by the asymmetric charge distribution in the protein. Thus, a protein can generate a lipid asymmetry without a direct binding interaction between lipid and protein. The generation of an asymmetry in charged lipid by this mechanism can also lead to a concomitant asymmetry in neutral lipids if deviations from ideality in the lipid mixture are taken into account. It is shown that regular solution theory applied to the lipid phase predicts an asymmetry in all components of a ternary mixture as long as one component is electrostatically oriented according to the mechanism mentioned above. The resulting asymmetry is not strongly salt dependent. The mechanism quantitatively accounts for the experimentally determined phospholipid asymmetry in the rod outer segment disc membrane of the vertebrate photoreceptor.
摘要
已表明，膜中带电荷脂质的跨膜不对称平衡是由膜中定向的双极蛋白质引起的。产生这种不对称的基本相互作用存在于脂质分子与由蛋白质中不对称电荷分布产生的跨膜电位之间。因此，蛋白质可以在脂质与蛋白质之间没有直接结合相互作用的情况下产生脂质不对称。如果考虑到脂质混合物中与理想状态的偏差，通过这种机制产生的带电荷脂质不对称也可能导致中性脂质的伴随不对称。结果表明，应用于脂质相的正规溶液理论预测，只要一种组分根据上述机制进行静电定向，三元混合物的所有组分都会出现不对称。所产生的不对称对盐的依赖性不强。该机制定量解释了脊椎动物光感受器杆状外段盘膜中实验测定的磷脂不对称现象。
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