[多瘤病毒大T抗原基因转染大鼠胚胎成纤维细胞后稳定细胞系的分离]
[Isolation of a stable cell line after transfection of rat embryo fibroblasts by the gene of the polyomavirus large T antigen].
作者信息
Komissarova E V, Revazova E S, Kiselev F L
出版信息
Biull Eksp Biol Med. 1988 Mar;105(3):347-9.
PMID:2832020
Abstract
A stable cell line REF(LT) was established upon transfection of DNA plasmid containing a large T gene of polyoma virus. REF(LT) cells grow in a monolayer, their growth depends on the underlayer, they are non-carcinogenic. The dependence of cellular growth on serum factors is decreased.
摘要
通过转染含有多瘤病毒大T基因的DNA质粒建立了稳定的细胞系REF(LT)。REF(LT)细胞呈单层生长，其生长依赖于下层细胞，无致癌性。细胞生长对血清因子的依赖性降低。
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