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[多瘤病毒大T抗原基因转染大鼠胚胎成纤维细胞后稳定细胞系的分离]

[Isolation of a stable cell line after transfection of rat embryo fibroblasts by the gene of the polyomavirus large T antigen].

作者信息

Komissarova E V, Revazova E S, Kiselev F L

出版信息

Biull Eksp Biol Med. 1988 Mar;105(3):347-9.

PMID:2832020
Abstract

A stable cell line REF(LT) was established upon transfection of DNA plasmid containing a large T gene of polyoma virus. REF(LT) cells grow in a monolayer, their growth depends on the underlayer, they are non-carcinogenic. The dependence of cellular growth on serum factors is decreased.

摘要

通过转染含有多瘤病毒大T基因的DNA质粒建立了稳定的细胞系REF(LT)。REF(LT)细胞呈单层生长,其生长依赖于下层细胞,无致癌性。细胞生长对血清因子的依赖性降低。

相似文献

1
[Isolation of a stable cell line after transfection of rat embryo fibroblasts by the gene of the polyomavirus large T antigen].[多瘤病毒大T抗原基因转染大鼠胚胎成纤维细胞后稳定细胞系的分离]
Biull Eksp Biol Med. 1988 Mar;105(3):347-9.
2
[Production of a cell line of embryonic rat fibroblasts after transfection with the DNA from the early region of simian adenovirus SA7].[用猴腺病毒SA7早期区域的DNA转染后产生胚胎大鼠成纤维细胞系]
Biull Eksp Biol Med. 1986 Feb;101(2):190-2.
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[The effect of Ha-ras oncogene on cells immortalized by the gene for polyomavirus large T-antigen].[哈-拉斯癌基因对由多瘤病毒大T抗原基因永生化的细胞的影响]
Mol Gen Mikrobiol Virusol. 1990 Feb(2):19-22.
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Establishment of cell lines from primary cultures by transfection with SV40 large T antigen gene.
Prog Clin Biol Res. 1987;253:101-9.
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Invasiveness and metastatic capability of rat fibroblast-like cells before and after transfection with immortalizing and transforming genes.大鼠成纤维样细胞在转染永生化和转化基因前后的侵袭性和转移能力。
Cancer Res. 1986 Sep;46(9):4787-95.
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Polyomavirus large T antigen overcomes p53 dependent growth arrest.多瘤病毒大T抗原克服p53依赖性生长停滞。
Oncogene. 1997 Apr 24;14(16):1923-31. doi: 10.1038/sj.onc.1201025.
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Effect on polyomavirus T-antigen function of mutations in a conserved leucine-rich segment of the DnaJ domain.DnaJ结构域保守富含亮氨酸片段中的突变对多瘤病毒T抗原功能的影响。
J Virol. 2001 Mar;75(5):2253-61. doi: 10.1128/JVI.75.5.2253-2261.2001.
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The three transforming regions of SV40 T antigen are required for immortalization of primary mouse embryo fibroblasts.SV40 T抗原的三个转化区域是原代小鼠胚胎成纤维细胞永生化所必需的。
Oncogene. 1995 Dec 7;11(11):2295-302.
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Transformation of rat-embryo immortalized fibroblasts by the E6-E7 region of human papillomavirus type 18.
Biomed Sci. 1991;2(3):305-8.
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Distinct amounts of polyomavirus large T antigen are required for different functions of the protein.多瘤病毒大T抗原的不同功能需要不同数量的该蛋白。
Oncogene. 1993 May;8(5):1277-83.

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