Sitaras N M, Sariban E, Bravo M, Pantazis P, Antoniades H N
Division of Biological Sciences, Harvard School of Public Health, Boston, Massachusetts.
Cancer Res. 1988 Apr 1;48(7):1930-5.
Prostate carcinoma cell lines DU-145 and PC-3 express both platelet derived growth factor (PDGF)-1 and PDGF-2/sis genes. Concomitantly, these cells synthesize and secrete PDGF-like proteins, as judged by indirect immunofluorescence and by direct immunoprecipitation with specific PDGF antiserum. Conditioned media derived from DU-145 and PC-3 cells stimulated the incorporation of [3H]thymidine by 3T3 cells and competed with 125I-labeled PDGF for its binding to cell surface receptors of 3T3 cells. The biological activity was stable to heating at 100 degrees C for 10 min, sensitive to reducing agents, and neutralized by the IgG fraction of PDGF antiserum, properties similar to those of authentic PDGF. Both DU-145 and PC-3 cell lines appear to lack receptors for PDGF as indicated by their inability to mitogenically respond to PDGF and receptor binding of 125I-labeled PDGF. Production of PDGF-like proteins by human prostate carcinoma cells may play an important role in a paracrine mode in the organization of the extracellular matrix of the malignant tissue.
前列腺癌细胞系DU - 145和PC - 3同时表达血小板衍生生长因子（PDGF）-1和PDGF - 2/sis基因。同时，通过间接免疫荧光和用特异性PDGF抗血清进行直接免疫沉淀判断，这些细胞合成并分泌PDGF样蛋白。来自DU - 145和PC - 3细胞的条件培养基刺激3T3细胞掺入[3H]胸腺嘧啶，并与125I标记的PDGF竞争其与3T3细胞表面受体的结合。该生物活性在100℃加热10分钟后稳定，对还原剂敏感，并被PDGF抗血清的IgG组分中和，这些特性与天然PDGF相似。DU - 145和PC - 3细胞系似乎都缺乏PDGF受体，这表现为它们对PDGF无促有丝分裂反应以及不能结合125I标记的PDGF受体。人前列腺癌细胞产生PDGF样蛋白可能在恶性组织细胞外基质组织的旁分泌模式中起重要作用。
