Yamaguchi Takashi, Ito Masahiko, Kuroda Keiji, Takeda Satoru, Tanaka Atsushi
Saint Mother Obstetrics and Gynecology Clinic, Institute for ART, Fukuoka 807-0825, Japan; Department of Obstetrics and Gynecology, Juntendo University School of Medicine, Tokyo 113-0033, Japan.
Department of Virology and Parasitology, Hamamatsu University School of Medicine, Hamamatsu 431-3192, Japan.
Cell Calcium. 2017 Jul;65:22-30. doi: 10.1016/j.ceca.2017.03.002. Epub 2017 Mar 6.
Phospholipase C-zeta (PLCZ1), a strong candidate of egg-activating sperm factor, can induce Ca oscillations and cause egg activation. For the application of PLCZ1 to clinical use, we examined the pattern of Ca responses and developmental rate by comparing PLCZ1 RNA injection methods with the other current methods, such as cytosolic aspiration, electrical stimulation and ionomycin treatment in human oocytes. We found that the pattern of Ca oscillations after PLCZ1 RNA injection exhibited similar characteristics to that after ICSI treatment. We also determined the optimal concentration of human PLCZ1 RNA to activate the human oocytes. Our findings suggest that human PLCZ1 RNA is a better therapeutic agent to rescue human oocytes from failed activation, leading to normal and efficient development.
磷脂酶Cζ（PLCZ1）是卵子激活精子因子的有力候选者，可诱导钙离子振荡并引起卵子激活。为了将PLCZ1应用于临床，我们通过比较PLCZ1 RNA注射方法与目前其他方法（如人卵母细胞的胞质抽吸、电刺激和离子霉素处理），研究了钙离子反应模式和发育率。我们发现，注射PLCZ1 RNA后的钙离子振荡模式与卵胞浆内单精子注射（ICSI）处理后的模式具有相似特征。我们还确定了激活人卵母细胞的人PLCZ1 RNA的最佳浓度。我们的研究结果表明，人PLCZ1 RNA是一种更好的治疗剂，可将人卵母细胞从激活失败中挽救出来，从而实现正常且高效的发育。
