Yunovitz H, Yawetz A
Institute for Nature Conservation Research, George S. Wise Faculty of Life Sciences, Tel Aviv University, Ramat Aviv, Israel.
FEBS Lett. 1988 Mar 28;230(1-2):105-8. doi: 10.1016/0014-5793(88)80651-3.
The delta-endotoxin produced by Bacillus thuringiensis ssp. entomocidus induced the release of encapsulated [14C]sucrose from reverse-phase vesicles composed of phosphatidylcholine and cholesterol. No such release was detected when the phospholipid component of the vesicles was either phosphatidylethanolamine, phosphatidylglycerol, or sphingomyelin. The toxin-induced release was competitively inhibited by negatively charged organic ions while positively charged organic ions, apart from choline chloride, had no such effect. The existence of a polar head group in the phospholipid as well as intermolecular hydrogen bonding at the membrane surface, was found to be of major importance in the toxin-liposome interaction.
苏云金芽孢杆菌昆虫亚种产生的δ-内毒素可诱导由磷脂酰胆碱和胆固醇组成的反相囊泡释放包裹的[14C]蔗糖。当囊泡的磷脂成分是磷脂酰乙醇胺、磷脂酰甘油或鞘磷脂时，未检测到这种释放。毒素诱导的释放受到带负电荷的有机离子的竞争性抑制，而带正电荷的有机离子（除氯化胆碱外）则没有这种作用。发现磷脂中极性头部基团的存在以及膜表面的分子间氢键在毒素-脂质体相互作用中至关重要。
