Iarkov S P, Tret'iakov S I, Zlobin V N
Biofizika. 1993 Jul-Aug;38(4):672-7.
We studied release of the fluorescent calcein marker from egg-lecithin liposomes under action of intact delta-endotoxin from Bacillus thuringiensis var. israilensis, and hydrolysed preparation of delta-endotoxin from Bacillus thuringiensis var. kurstaki together with the motional parameters of 5- and 12-doxyl stearic radical probes in the liposomal membrane. The yield of the non-penetrating fluorescent marker was shown to be a sensitive indicator of the disturbance of the membrane barrier function.
我们研究了苏云金芽孢杆菌以色列变种完整的δ-内毒素作用下,荧光素标记物从卵磷脂脂质体中的释放情况,以及苏云金芽孢杆菌库尔斯塔克变种δ-内毒素水解制剂与脂质体膜中5-和12-硬脂酰氧基硬脂酸自由基探针的运动参数。结果表明,非穿透性荧光标记物的产量是膜屏障功能紊乱的敏感指标。