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[苏云金芽孢杆菌δ-内毒素作用下脂质体磷脂膜通透性的变化]

[Change in the permeability of liposome phospholipid membranes under the effect of Bacillus thuringiensis delta-endotoxins].

作者信息

Iarkov S P, Tret'iakov S I, Zlobin V N

出版信息

Biofizika. 1993 Jul-Aug;38(4):672-7.

PMID:8364069
Abstract

We studied release of the fluorescent calcein marker from egg-lecithin liposomes under action of intact delta-endotoxin from Bacillus thuringiensis var. israilensis, and hydrolysed preparation of delta-endotoxin from Bacillus thuringiensis var. kurstaki together with the motional parameters of 5- and 12-doxyl stearic radical probes in the liposomal membrane. The yield of the non-penetrating fluorescent marker was shown to be a sensitive indicator of the disturbance of the membrane barrier function.

摘要

我们研究了苏云金芽孢杆菌以色列变种完整的δ-内毒素作用下,荧光素标记物从卵磷脂脂质体中的释放情况,以及苏云金芽孢杆菌库尔斯塔克变种δ-内毒素水解制剂与脂质体膜中5-和12-硬脂酰氧基硬脂酸自由基探针的运动参数。结果表明,非穿透性荧光标记物的产量是膜屏障功能紊乱的敏感指标。

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